Human C-reactive protein activates monocyte-derived dendritic cells and induces dendritic cell-mediated T-cell activation

Objective-Recent studies proposed a pathogenic role for C-reactive protein (CRP), an independent predictor of cardiovascular disease (CVD), in atherosclerosis. Therefore, we tested whether CRP may modulate dendritic cell (DC) function, because these professional antigen-presenting cells have been implicated in atherogenesis. Methods and Results-Human monocyte-derived immature DCs were cultured with human CRP (0 to 60 mu g/mL) for 24 hours. Thereafter, activation markers were measured by flow-cytometry and DCs were cocultured with CFSE-labeled lymphocytes to measure T-cell proliferation and interferon (IFN)-gamma secretion after 8 days. Exposure to 60 mu g/mL CRP (n = 5) induced an activated cell morphology and significant (CD40 increase MFI 5.23 +/- 0.28, P < 0.01 paired t test; CD80 6.18 +/- 0.51, P < 0.01) to modest (CD83 1.38 +/- 0.17, P < 0.05, CCR7 1.60 +/- 0.29, P < 0.05) upregulation of DC activation markers. The expression of CD86 and HLA-DR was high, but not affected. T-lymphocytes incubated with CRP-pulsed DCs displayed increased IFN-gamma secretion and proliferation (P < 0.001). DC activation was concentration-dependent and detected from 2 mu g/mL CRP; the maximum effect was equivalent to that seen with 0.1 mu g/mL lipopolysaccharide (LPS). Polymyxin B abolished the LPS response, without influencing CRP effects. Finally, immunohistochemistry could demonstrate DC/CRP colocalization in human atherosclerotic lesions. Conclusions-These findings suggest that CRP in plaques or found circulating in CVD patients can influence DC function during atherogenesis.