Nuclear localization of prostaglandin E2 receptors

被引：207
作者：
Bhattacharya, M
Peri, KG
Almazan, G
Ribeiro-Da-Silva, A
Shichi, H
Durocher, Y
Abramovitz, M
Hou, X
Varma, DR
Chemtob, S
机构：
[1] Hop St Justine, Montreal, PQ H3T 1C5, Canada
[2] Dept Pediat, Montreal, PQ H3T 1C5, Canada
[3] McGill Univ, Dept Pharmacol & Therapeut, Montreal, PQ H3G 1Y6, Canada
[4] Merck Frosst Labs, Pointe Claire, PQ H9R 4P8, Canada
[5] Wayne State Univ, Dept Ophthalmol, Detroit, MI 48201 USA
[6] Biotechnol Res Inst, Montreal, PQ H4P 2R2, Canada
来源：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1998年 / 95卷 / 26期
关键词：
D O I：
10.1073/pnas.95.26.15792
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Prostaglandin E-2 receptors (EP) were detected by radioligand binding in nuclear fractions isolated from porcine brain and myometrium. Intracellular localization by immunocytofluorescence revealed perinuclear localization of EPs in porcine cerebral microvascular endothelial cells. Nuclear association of EP1 was also found in fibroblast Swiss 3T3 cells stably overexpressing EP1 and in human embryonic kidney 293 (Epstein-Barr virus-encoded nuclear antigen) cells expressing EP1 fused to green fluorescent protein. High-resolution immunostaining of EP1 revealed their presence in the nuclear envelope of isolated (cultured) endothelial cells and in situ in brain (cortex) endothelial cells and neurons. Stimulation of these nuclear receptors modulate nuclear calcium and gene transcription.
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页码：15792 / 15797
页数：6
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