Visualizing nitric oxide-dependent HIF-1 activity under hypoxia with a lipid droplet-targeting fluorescent probe

被引：30

作者：

Pan, Ying-Hao ^{[1
]}

Chen, Xiao-Xiao ^{[1
]}

Dong, Lei ^{[1
]}

Shao, Na ^{[1
]}

Niu, Li-Ya ^{[1
]}

Yang, Qing-Zheng ^{[1
]}

机构：

[1] Beijing Normal Univ, Coll Chem, Key Lab Radiopharmaceut, Minist Educ, Beijing 100875, Peoples R China

来源：

CHINESE CHEMICAL LETTERS | 2021年 / 32卷 / 12期

基金：

中国国家自然科学基金;

关键词：

Bodipy; Fluorescent probe; HIF-1; Lipid droplet; Nitric oxide; FLUOROGENIC PROBES; CELLS; TRACKING; NO;

D O I：

10.1016/j.cclet.2021.06.024

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Evaluating the correlation between hypoxia inducible factor 1 (HIF-1) and nitric oxide (NO) generated under hypoxia is of great significance. In this work, we developed a fluorescent probe for the monitor of HIF-1 activity influenced by NO under hypoxia in hepatoma cells with dual-targeting for hepatocyte and lipid droplet (LD). The probe shows excellent selectivity to NO and high sensitivity with 600 0-fold fluorescence enhancement. Live cell imaging experiments revealed the probe's capability of imaging exogenous and endogenous NO with specific in LDs of HepG2 cells. For cells under hypoxia, HIF-1 induced LD level is observed to correlate with NO level. This work provides the in-situ visualization of NOdependent HIF-1 upregulation through LD accumulation. (C) 2021 Published by Elsevier B.V. on behalf of Chinese Chemical Society and Institute of Materia Medica, Chinese Academy of Medical Sciences.

引用

页码：3895 / 3898

页数：4