Expression of distinct RNAs from 3′ untranslated regions

被引：158

作者：

Mercer, Tim R. ^{[1
]}

Wilhelm, Dagmar ^{[1
]}

Dinger, Marcel E. ^{[1
]}

Solda, Giulia ^{[1
,2
]}

Korbie, Darren J. ^{[1
]}

Glazov, Evgeny A. ^{[1
,3
]}

Vy Truong ^{[1
]}

Schwenke, Maren ^{[1
]}

Simons, Cas ^{[1
,4
]}

Matthaei, Klaus I. ^{[5
,6
]}

Saint, Robert ^{[7
,8
]}

Koopman, Peter ^{[1
]}

Mattick, John S. ^{[1
]}

机构：

[1] Univ Queensland, Inst Mol Biosci, Brisbane, Qld 4072, Australia

[2] Univ Milan, Dipartimento Biol & Genet Sci Med, I-20122 Milan, Italy

[3] Univ Queensland, Diamantina Inst Canc Immunol & Metab Med, Brisbane, Qld 4102, Australia

[4] Univ Queensland, Queensland Facil Adv Bioinformat, Brisbane, Qld 4072, Australia

[5] Australian Natl Univ, John Curtin Sch Med Res, Canberra, ACT 2601, Australia

[6] King Saud Univ, Dept Anat, Stem Cell Unit, Riyadh, Saudi Arabia

[7] Australian Natl Univ, Res Sch Biol Sci, Canberra, ACT 2601, Australia

[8] Univ Melbourne, Fac Sci, Parkville, Vic 3010, Australia

来源：

NUCLEIC ACIDS RESEARCH | 2011年 / 39卷 / 06期

基金：

澳大利亚研究理事会; 英国医学研究理事会;

关键词：

LONG NONCODING RNAS; HUMAN GENOME; TRANSLATIONAL CONTROL; MYELOID-LEUKEMIA; MESSENGER-RNAS; DIFFERENTIATION; GENE; TRANSCRIPTS; DATABASE; CELLS;

D O I：

10.1093/nar/gkq1158

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The 3' untranslated regions (3'UTRs) of eukaryotic genes regulate mRNA stability, localization and translation. Here, we present evidence that large numbers of 3'UTRs in human, mouse and fly are also expressed separately from the associated protein-coding sequences to which they are normally linked, likely by post-transcriptional cleavage. Analysis of CAGE (capped analysis of gene expression), SAGE (serial analysis of gene expression) and cDNA libraries, as well as microarray expression profiles, demonstrate that the independent expression of 3'UTRs is a regulated and conserved genome-wide phenomenon. We characterize the expression of several 3'UTR-derived RNAs (uaRNAs) in detail in mouse embryos, showing by in situ hybridization that these transcripts are expressed in a cell- and subcellular-specific manner. Our results suggest that 3'UTR sequences can function not only in cis to regulate protein expression, but also intrinsically and independently in trans, likely as noncoding RNAs, a conclusion supported by a number of previous genetic studies. Our findings suggest novel functions for 3'UTRs, as well as caution in the use of 3'UTR sequence probes to analyze gene expression.

引用

页码：2393 / 2403

页数：11

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