Antiproliferative Activity and Apoptosis Induction of Eucalyptus Citriodora Resin and Its Major Bioactive Compound in Melanoma B16F10 Cells

被引：30

作者：

Duh, Pin-Der ^{[2
]}

Chen, Zong-Tsi ^{[3
]}

Lee, Shwu-Woan ^{[3
]}

Lin, Tsuey-Pin ^{[4
]}

Wang, Ya-Ting ^{[1
]}

Yen, Wen-Jye ^{[2
]}

Kuo, Ling-Feng ^{[1
]}

Chu, Heuy-Ling ^{[2
]}

机构：

[1] Kuo Gen Hosp, Taninan, Taiwan

[2] Chia Nan Univ Pharm & Sci, Dept Food Sci & Technol, Tainan, Taiwan

[3] Chia Nan Univ Pharm & Sci, Dept Med Chem, Tainan, Taiwan

[4] Chia Nan Univ Pharm & Sci, Dept Hlth & Nutr, Tainan, Taiwan

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2012年 / 60卷 / 32期

关键词：

B16F10 melanoma cell; Eucalyptus citriodora resin; oxidative stress; apoptosis; 6-[1-(p-hydroxy-phenyl)ethyl]-7-O-methyl aromadendrin; antiproliferation; CANCER CELLS; IN-VITRO; ACID; ANTIOXIDANT; MECHANISMS; GROWTH; CERVIX; LUNG; TEA;

D O I：

10.1021/jf301068z

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

Antiproliferative activity and apoptosis induction of ethyl acetate of Eucalyptus citriodora resin (EAEER), and its major bioactive compound in melanoma B16F10 cells were investigated. 6-[1-(p-Hydroxy-phenyl)ethyl]-7-O-methyl aromadendrin (HEMA), a flavanol derivative, was isolated from EAEER and identified on the basis of its mass and NMR spectra. The results from MTT assay showed high antiproliferative effects of EAEER and HEMA on B16F10 cells. Moreover, EAEER- and HEMA-induced cell apoptosis was association with the decrease in the mitochondrial transmembrane potentials (Delta psi(m)), increase in Bax/Bcl-2 ratio, and activation of caspase-3. Cells treated with EAEER and HEMA generated intracellular reactive oxygen species (ROS) and nitric oxide (NO), indicating that ROS and RNS play important roles in the induction of apoptosis in B16F10 cells. Taken together, EAEER and its major bioactive compound, HEMA, inhibited the proliferation of B16F10 cells via apoptosis and may be a potential antimelanoma agent.

引用

页码：7866 / 7872

页数：7

共 25 条

[1] Polysaccharides isolated from Phellinus gilvus inhibit melanoma growth in mice [J].