Ozone and aging up-regulate type II metacaspase gene expression and global metacaspase activity in the leaves of field-grown maize (Zea mays L.) plants

被引：28

作者：

Ahmad, Rafiq ^{[1
]}

Zuily-Fodil, Yasmine ^{[1
]}

Passaquet, Chantal ^{[1
]}

Bethenod, Olivier ^{[2
]}

Roche, Romain ^{[2
]}

Repellin, Anne ^{[1
]}

机构：

[1] Univ Paris Est Creteil, UMR Bioemco 7618, Equipe IBIOS, F-94010 Creteil, France

[2] INRA, UMR Environm & Grandes Cultures 1091, F-78850 Thiverval Grignon, France

来源：

CHEMOSPHERE | 2012年 / 87卷 / 07期

关键词：

Tropospheric O-3; Type II metacaspases; Zea mays L; RT-qPCR; Global metacaspase activities; PROGRAMMED CELL-DEATH; ARABIDOPSIS; PROTEINS; BETA-1,3-GLUCANASE; CHITINASE; RESPONSES; POPLAR;

D O I：

10.1016/j.chemosphere.2011.12.081

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

Maize plants (Zea mays L cv. NK Perform) were exposed to O-3-enriched air, using a new field fumigation system. Transcriptional changes for three type II-metacaspase genes were studied in the leaves (ranks 10 and 12), using quantitative real-time PCR. Global metacaspase activity was measured using metacaspase-specific synthetic tripeptide Boc-GRR-AMC. Aging had little effect on mRNA accumulation whereas four to six-fold increases were observed for the most O-3-responsive type II metacaspase genes, in the older leaves 10. Global metacaspase activity increased by 257% and 333% in leaves 12 and 10, respectively, in response to the highest cumulated concentration. In non-fumigated plants, metacaspase activity progressively increased over the course of the experiment and always was higher in the older leaves 10. Together, these results suggest that metacaspase-mediated proteolysis is a crucial step in leaf responses to both O-3 and age-mediated senescence. (C) 2012 Elsevier Ltd. All rights reserved.

引用

页码：789 / 795

页数：7

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