A robust and quantitative assay platform for multiplexed, high throughput screening of protein kinase inhibitors

被引：11

作者：

Lee, Jieon ^{[1
]}

Park, Il-Soo ^{[1
]}

Park, Ginam ^{[1
]}

Cho, Kyukwang ^{[2
]}

Park, Hee-Sung ^{[2
]}

Min, Dal-Hee ^{[1
]}

机构：

[1] Seoul Natl Univ, Dept Chem, Inst Basic Sci, Ctr RNA Res, Seoul 151747, South Korea

[2] Korea Adv Inst Sci & Technol, Dept Chem, Taejon 305701, South Korea

来源：

CHEMICAL COMMUNICATIONS | 2016年 / 52卷 / 81期

基金：

新加坡国家研究基金会;

关键词：

GRAPHENE OXIDE; MASS-SPECTROMETRY; PHOSPHORYLATION PROTECTION; HYBRID MATERIALS; CELLS; PHOSPHOPROTEOME; INFLAMMATION; BIOMOLECULES; ACTIVATION; BIOSENSOR;

D O I：

10.1039/c6cc05834e

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

We present a new platform for multiplexed protein kinase activity assay using TiO2 decorated graphene oxide (GO), which is applicable to high throughput inhibitor screening. On the basis of the strong affinity of TiO2 for the phosphate group and the fluorescence quenching capability of GO, phosphorylation of substrates by protein kinases was quantitatively measured in a short time.

引用

页码：12112 / 12115

页数：4

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