Serum surfactant protein D is increased in acute and chronic inflammation in mice

被引:72
作者
Fujita, M
Shannon, JM
Ouchi, H
Voelker, DR
Nakanishi, Y
Mason, RJ
机构
[1] Kyushu Univ, Grad Sch Med Sci, Res Inst Dis Chest, Higashi Ku, Fukuoka 8128582, Japan
[2] Natl Jewish Med & Res Ctr, Dept Med, Denver, CO USA
[3] Cincinnati Childrens Hosp, Med Ctr, Dept Pediat, Div Pulm Biol, Cincinnati, OH USA
关键词
surfactant protein; TNF-alpha; bleomycin; inflammation; CC-10;
D O I
10.1016/j.cyto.2005.02.006
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Surfactant protein A (SP-A) and surfactant protein D (SP-D) are important components of innate immunity that can modify the inflammatory response. However, alterations and regulation of SP-A and SP-D in acute and chronic inflammation are not well defined. In addition, serum SP-D may serve as a biomarker of lung inflammation. We determined the expression of SP-A and SP-D in murine models. To study acute inflammation, we instilled bleomycin intrabronchially. To study chronic lung inflammation, we used a transgenic mouse that overexpresses tumor necrosis factor (TNF)-alpha. under the control of the SP-C promoter. These mice have a chronic mononuclear cell infiltration, airspace enlargement, pulmonary hypertension, and focal pulmonary fibrosis. In acute inflammation model, levels of mRNA for all surfactant proteins were reduced after bleomycin administration. However, serum SP-D was increased from days 7 to 28 after instillation. In chronic inflammation model, SP-D mRNA expression was increased, whereas the expression of SP-A, SP-B and SP-C was reduced. Both serum and lung SP-D concentrations were increased in chronic lung inflammation. These data clarified profile of SP-A and SP-D in acute and chronic inflammation and indicated that serum SP-D can serve as a biomarker of lung inflammation in both acute and chronic lung injury in mice. (c) 2005 Elsevier Ltd. All rights reserved.
引用
收藏
页码:25 / 33
页数:9
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