Generation of Induced Pluripotent Stem Cells from Urine

被引：331

作者：

Zhou, Ting ^{[1
]}

Benda, Christina ^{[1
,2
]}

Duzinger, Sarah ^{[2
]}

Huang, Yinghua ^{[1
]}

Li, Xingyan ^{[1
]}

Li, Yanhua ^{[1
]}

Guo, Xiangpeng ^{[1
]}

Cao, Guokun ^{[1
]}

Chen, Shen ^{[1
]}

Hao, Lili ^{[3
]}

Chan, Yau-Chi ^{[4
]}

Ng, Kwong-Man ^{[4
]}

Ho, Jenny Cy ^{[4
]}

Wieser, Matthias ^{[2
,5
]}

Wu, Jiayan ^{[3
]}

Redl, Heinz ^{[6
]}

Tse, Hung-Fat ^{[4
]}

Grillari, Johannes ^{[2
,7
]}

Grillari-Voglauer, Regina ^{[2
,7
]}

Pei, Duanqing ^{[1
]}

Esteban, Miguel A. ^{[1
]}

机构：

[1] Chinese Acad Sci, Stem Cell & Canc Biol Grp, Guangzhou Inst Biomed & Hlth,Key Lab Regenerat Bi, S China Inst Stem Cell Biol & Regenerat Med, Guangzhou, Guangdong, Peoples R China

[2] Univ Nat Resources & Life Sci, Dept Biotechnol, Vienna, Austria

[3] Chinese Acad Sci, Key Lab Genome Sci & Informat, Beijing Inst Genom, Beijing, Peoples R China

[4] Univ Hong Kong, Div Cardiol, Dept Med, Li Ka Shing Fac Med,Queen Mary Hosp, Pokfulam, Hong Kong, Peoples R China

[5] ACIB, Vienna, Austria

[6] Austrian Cluster Tissue Regenerat, Ludwig Boltzmann Inst Clin & Expt Traumatol, Vienna, Austria

[7] Evercyte GmbH, A-1190 Vienna, Austria

来源：

JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY | 2011年 / 22卷 / 07期

基金：

奥地利科学基金会;

关键词：

EPITHELIAL-CELLS; EFFICIENT;

D O I：

10.1681/ASN.2011010106

中图分类号：

R5 [内科学]; R69 [泌尿科学（泌尿生殖系疾病）];

学科分类号：

1002 ; 100201 ;

摘要：

Forced expression of selected transcription factors can transform somatic cells into embryonic stem cell (ESC)-like cells, termed induced pluripotent stem cells (iPSCs). There is no consensus regarding the preferred tissue from which to harvest donor cells for reprogramming into iPSCs, and some donor cell types may be more prone than others to accumulation of epigenetic imprints and somatic cell mutations. Here, we present a simple, reproducible, noninvasive method for generating human iPSCs from renal tubular cells present in urine. This procedure eliminates many problems associated with other protocols, and the resulting iPSCs display an excellent ability to differentiate. These data suggest that urine may be a preferred source for generating iPSCs.

引用

页码：1221 / 1228

页数：8

共 23 条

[1] Efficient and rapid generation of induced pluripotent stem cells from human keratinocytes [J].

Aasen, Trond ;

Raya, Angel ;

Barrero, Maria J. ;

Garreta, Elena ;

Consiglio, Antonella ;

Gonzalez, Federico ;

Vassena, Rita ;

Bilic, Josipa ;

Pekarik, Vladimir ;

Tiscornia, Gustavo ;

Edel, Michael ;

Boue, Stephanie ;

Izpisua Belmonte, Juan Carlos .

NATURE BIOTECHNOLOGY, 2008, 26 (11) :1276-1284

[2] Generation of Human Induced Pluripotent Stem Cells from Umbilical Cord Matrix and Amniotic Membrane Mesenchymal Cells [J].

Cai, Jinglei ;

Li, Wen ;

Su, Huanxing ;

Qin, Dajiang ;

Yang, Jiayin ;

Zhu, Fan ;

Xu, Jianyong ;

He, Wenzhi ;

Guo, Xiangpeng ;

Labuda, Krystyna ;

Peterbauer, Anja ;

Wolbank, Susanne ;

Zhong, Mei ;

Li, Zhiyuan ;

Wu, Wutian ;

So, Kwok-Fai ;

Redl, Heinz ;

Zeng, Lingwen ;

Esteban, Miguel Angel ;

Pei, Duanqing .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2010, 285 (15) :11227-11234

[3] Cultures of exfoliated epithelial cells from different locations of the human urinary tract and the renal tubular system [J].

Dörrenhaus, A ;

Müller, JIF ;

Golka, K ;

Jedrusik, P ;

Schulze, H ;

Föllmann, W .

ARCHIVES OF TOXICOLOGY, 2000, 74 (10) :618-626

[4] Vitamin C Enhances the Generation of Mouse and Human Induced Pluripotent Stem Cells [J].

Esteban, Miguel Angel ;

Wang, Tao ;

Qin, Baoming ;

Yang, Jiayin ;

Qin, Dajiang ;

Cai, Jinglei ;

Li, Wen ;

Weng, Zhihui ;

Chen, Jiekai ;

Ni, Su ;

Chen, Keshi ;

Li, Yuan ;

Liu, Xiaopeng ;

Xu, Jianyong ;

Zhang, Shiqiang ;

Li, Feng ;

He, Wenzhi ;

Labuda, Krystyna ;

Song, Yancheng ;

Peterbauer, Anja ;

Wolbank, Susanne ;

Redl, Heinz ;

Zhong, Mei ;

Cai, Daozhang ;

Zeng, Lingwen ;

Pei, Duanqing .

CELL STEM CELL, 2010, 6 (01) :71-79

[5] Generation of Induced Pluripotent Stem Cells from Human Cord Blood Using OCT4 and SOX2 [J].

Giorgetti, Alessandra ;

Montserrat, Nuria ;

Aasen, Trond ;

Gonzalez, Federico ;

Rodriguez-Piza, Ignacio ;

Vassena, Rita ;

Raya, Angel ;

Boue, Stephanie ;

Jose Barrero, Maria ;

Aran Corbella, Begona ;

Torrabadella, Marta ;

Veiga, Anna ;

Izpisua Belmonte, Juan Carlos .

CELL STEM CELL, 2009, 5 (04) :353-357

[6] Somatic coding mutations in human induced pluripotent stem cells [J].

Gore, Athurva ;

Li, Zhe ;

Fung, Ho-Lim ;

Young, Jessica E. ;

Agarwal, Suneet ;

Antosiewicz-Bourget, Jessica ;

Canto, Isabel ;

Giorgetti, Alessandra ;

Israel, Mason A. ;

Kiskinis, Evangelos ;

Lee, Je-Hyuk ;

Loh, Yuin-Han ;

Manos, Philip D. ;

Montserrat, Nuria ;

Panopoulos, Athanasia D. ;

Ruiz, Sergio ;

Wilbert, Melissa L. ;

Yu, Junying ;

Kirkness, Ewen F. ;

Izpisua Belmonte, Juan Carlos ;

Rossi, Derrick J. ;

Thomson, James A. ;

Eggan, Kevin ;

Daley, George Q. ;

Goldstein, Lawrence S. B. ;

Zhang, Kun .

NATURE, 2011, 471 (7336) :63-U76

[7] Generation of Induced Pluripotent Stem Cells from Human Cord Blood [J].

Haase, Alexandra ;

Olmer, Ruth ;

Schwanke, Kristin ;

Wunderlich, Stephanie ;

Merkert, Sylvia ;

Hess, Christian ;

Zweigerdt, Robert ;

Gruh, Ina ;

Meyer, Johann ;

Wagner, Stefan ;

Maier, Lars S. ;

Han, Dong Wook ;

Glage, Silke ;

Miller, Konstantin ;

Fischer, Philipp ;

Schoeler, Hans R. ;

Martin, Ulrich .

CELL STEM CELL, 2009, 5 (04) :434-441

[8] Copy number variation and selection during reprogramming to pluripotency [J].

Hussein, Samer M. ;

Batada, Nizar N. ;

Vuoristo, Sanna ;

Ching, Reagan W. ;

Autio, Reija ;

Narva, Elisa ;

Ng, Siemon ;

Sourour, Michel ;

Hamalainen, Riikka ;

Olsson, Cia ;

Lundin, Karolina ;

Mikkola, Milla ;

Trokovic, Ras ;

Peitz, Michael ;

Bruestle, Oliver ;

Bazett-Jones, David P. ;

Alitalo, Kari ;

Lahesmaa, Riitta ;

Nagy, Andras ;

Otonkoski, Timo .

NATURE, 2011, 471 (7336) :58-U67

[9]

Inoue CN, 2003, IN VITRO CELL DEV-AN, V39, P364

[10]

Iwatani H, 2010, J NEPHROL, V23, P143

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