Revealing the redox status in endoplasmic reticulum by a selenium fluorescence probe

被引：45

作者：

Zang, Shunping ^{[1
]}

Kong, Xiangxue ^{[1
]}

Cui, Jie ^{[1
]}

Su, Sa ^{[1
]}

Shu, Wei ^{[1
]}

Jing, Jing ^{[1
]}

Zhang, Xiaoling ^{[1
]}

机构：

[1] Beijing Inst Technol, Sch Chem & Chem Engn, Beijing Key Lab Photoelect Electrophoton Convers, Key Lab Cluster Sci,Minist Educ, Beijing 100081, Peoples R China

来源：

JOURNAL OF MATERIALS CHEMISTRY B | 2020年 / 8卷 / 13期

关键词：

PROTEIN DISULFIDE-ISOMERASE; HYPOCHLOROUS-ACID; REACTIVE OXYGEN; OXIDATIVE STRESS; LIVING CELLS; SE-BODIPY; GLUTATHIONE; PEROXYNITRITE; ANTIOXIDANTS; MECHANISMS;

D O I：

10.1039/c9tb02919b

中图分类号：

TB3 [工程材料学]; R318.08 [生物材料学];

学科分类号：

0805 ; 080501 ; 080502 ;

摘要：

As an important organelle, the endoplasmic reticulum (ER) participates in the synthesis and secretion of various proteins, glycogen, lipids and cholesterol in eukaryotic cells. In this work, an endoplasmic reticulum-targeted reversible fluorescent probe (ER-Se) was designed and synthesized. The probe, based on a selenide group, shows high sensitivity and good selectivity toward HClO (LOD = 0.85 mu M). In addition, the probe has reversible capability towards HClO/GSH. Most importantly, co-location experiment results indicated that the probe exhibited a great ability to target the endoplasmic reticulum. Furthermore, the probe was successfully applied to detect exogenous and endogenous HClO in ER and monitored the redox status changes during ER stress.

引用

页码：2660 / 2665

页数：6

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