Presence of bovine papillomavirus type 2 DNA and expression of the viral oncoprotein E5 in naturally occurring urinary bladder tumours in cows

被引：75
作者：
Borzacchiello, G
Iovane, G
Marcante, ML
Poggiali, F
Roperto, F
Roperto, S
Venuti, A
机构：
[1] Univ Naples Federico II, Dept Pathol & Anim Hlth, Fac Vet Med, I-80137 Naples, Italy
[2] Regina Elena Inst Canc Res, Virol Lab, Rome, Italy
来源：
JOURNAL OF GENERAL VIROLOGY | 2003年 / 84卷
关键词：
D O I：
10.1099/vir.0.19412-0
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Samples of neoplastic and normal urothelium were obtained from cows originating from areas of southern Italy, a region in which chronic enzootic haematuria is endemic and bracken fern infestation is widespread. Specimens were analysed for bovine papillomavirus type 2 (BPV-2) BPV-2 E5 expression and telomerase activity. A total of 46 of 60 tumours and 17 of 34 bladder mucosa samples harboured BPV-2 DNA. Analysis of a subset of samples showed E5 protein expression and telomerase activity in tumour tissue only. No normal samples positive BPV DNA showed E5 protein expression or telomerase activity, suggesting the presence of in a latent state. Taken together, these data on naturally occurring bovine bladder tumours corroborate the hypothesis of their virus origin.
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页码：2921 / 2926
页数：6
相关论文
共 38 条
  • [11] LOCALIZATION OF BOVINE PAPILLOMAVIRUS TYPE-1 E5 PROTEIN TO TRANSFORMED BASAL KERATINOCYTES AND PERMISSIVE DIFFERENTIATED CELLS IN FIBROPAPILLOMA TISSUE
    BURNETT, S
    JAREBORG, N
    DIMAIO, D
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (12) : 5665 - 5669
  • [12] THE SYNERGISM BETWEEN BOVINE PAPILLOMAVIRUS TYPE-4 AND QUERCETIN IS DEPENDENT ON THE TIMING OF EXPOSURE
    CAIRNEY, M
    CAMPO, MS
    [J]. CARCINOGENESIS, 1995, 16 (08) : 1997 - 2001
  • [13] EXPERIMENTAL REPRODUCTION OF THE PAPILLOMA-CARCINOMA COMPLEX OF THE ALIMENTARY CANAL IN CATTLE
    CAMPO, MS
    ONEIL, BW
    BARRON, RJ
    JARRETT, WFH
    [J]. CARCINOGENESIS, 1994, 15 (08) : 1597 - 1601
  • [14] Bovine papillomavirus and cancer
    Campo, MS
    [J]. VETERINARY JOURNAL, 1997, 154 (03) : 175 - 188
  • [15] CAMPO MS, 1990, ONCOGENE, V5, P303
  • [16] Expression of a transforming gene (E5) of bovine papillomavirus in sarcoids obtained from horses
    Carr, EA
    Théon, AP
    Madewell, BR
    Hitchcock, ME
    Schlegel, R
    Schiller, JT
    [J]. AMERICAN JOURNAL OF VETERINARY RESEARCH, 2001, 62 (08) : 1212 - 1217
  • [17] The expression of HPV-16 E5 protein in squamous neoplastic changes in the uterine cervix
    Chang, JL
    Tsao, YP
    Liu, DW
    Huang, SJ
    Lee, WH
    Chen, SL
    [J]. JOURNAL OF BIOMEDICAL SCIENCE, 2001, 8 (02) : 206 - 213
  • [18] Confer A.W., 2001, THOMSONS SPECIAL VET, P235
  • [19] The BPV-4 co-carcinogen quercetin induces cell cycle arrest and up-regulates transcription from the LCR of BPV-4
    Connolly, JA
    Morgan, IM
    Jackson, ME
    Campo, MS
    [J]. ONCOGENE, 1998, 16 (21) : 2739 - 2746
  • [20] THE HUMAN PAPILLOMAVIRUS TYPE-6 AND 16-E5 PROTEINS ARE MEMBRANE-ASSOCIATED PROTEINS WHICH ASSOCIATE WITH THE 16-KILODALTON PORE-FORMING PROTEIN
    CONRAD, M
    BUBB, VJ
    SCHLEGEL, R
    [J]. JOURNAL OF VIROLOGY, 1993, 67 (10) : 6170 - 6178
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