Presence of bovine papillomavirus type 2 DNA and expression of the viral oncoprotein E5 in naturally occurring urinary bladder tumours in cows

被引：76
作者：
Borzacchiello, G
Iovane, G
Marcante, ML
Poggiali, F
Roperto, F
Roperto, S
Venuti, A
机构：
[1] Univ Naples Federico II, Dept Pathol & Anim Hlth, Fac Vet Med, I-80137 Naples, Italy
[2] Regina Elena Inst Canc Res, Virol Lab, Rome, Italy
来源：
JOURNAL OF GENERAL VIROLOGY | 2003年 / 84卷
关键词：
D O I：
10.1099/vir.0.19412-0
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Samples of neoplastic and normal urothelium were obtained from cows originating from areas of southern Italy, a region in which chronic enzootic haematuria is endemic and bracken fern infestation is widespread. Specimens were analysed for bovine papillomavirus type 2 (BPV-2) BPV-2 E5 expression and telomerase activity. A total of 46 of 60 tumours and 17 of 34 bladder mucosa samples harboured BPV-2 DNA. Analysis of a subset of samples showed E5 protein expression and telomerase activity in tumour tissue only. No normal samples positive BPV DNA showed E5 protein expression or telomerase activity, suggesting the presence of in a latent state. Taken together, these data on naturally occurring bovine bladder tumours corroborate the hypothesis of their virus origin.
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页码：2921 / 2926
页数：6
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