Novel Picornavirus in Turkey Poults with Hepatitis, California, USA

被引：56
作者：
Honkavuori, Kirsi S.
Shivaprasad, H. L. [2 ]
Briese, Thomas [1 ]
Street, Craig
Hirschberg, David L.
Hutchison, Stephen K. [3 ]
Lipkin, W. Ian
机构：
[1] Columbia Univ, Ctr Infect & Immun, New York, NY 10032 USA
[2] Calif Anim Hlth & Food Safety Lab Syst, Tulare, CA USA
[3] 454 Life Sci, Branford, CT USA
来源：
EMERGING INFECTIOUS DISEASES | 2011年 / 17卷 / 03期
基金：
美国国家卫生研究院;
关键词：
MOLECULAR CHARACTERIZATION; VIRAL-HEPATITIS; VIRUS; IDENTIFICATION;
D O I：
10.3201/eid1703.101410
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
To identify a candidate etiologic agent for turkey viral hepatitis, we analyzed samples from diseased turkey poults from 8 commercial flocks in California, USA, that were collected during 2008-2010. High-throughput pyrosequencing of RNA from livers of poults with turkey viral hepatitis (TVH) revealed picornavirus sequences. Subsequent cloning of the approximate to 9-kb genome showed an organization similar to that of picornaviruses with conservation of motifs within the P1, P2, and P3 genome regions, but also unique features, including a 1.2-kb sequence of unknown function at the junction of P1 and P2 regions. Real-time PCR confirmed viral RNA in liver, bile, intestine, serum, and cloacal swab specimens from diseased poults. Analysis of liver by in situ hybridization with viral probes and immunohistochemical testing of serum demonstrated viral nucleic acid and protein in livers of diseased poults. Molecular, anatomic, and immunologic evidence suggests that TVH is caused by a novel picornavirus, tentatively named turkey hepatitis virus.
引用
收藏
页码：480 / 487
页数：8
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