Reprogramming somatic cells towards pluripotency by defined factors

被引：101
作者：
Lewitzky, Marc [1 ]
Yamanaka, Shinya [1 ]
机构：
[1] Kyoto Univ, Inst Frontier Med Sci, Sakyo Ku, Kyoto 6068507, Japan
来源：
CURRENT OPINION IN BIOTECHNOLOGY | 2007年 / 18卷 / 05期
基金：
日本学术振兴会;
关键词：
D O I：
10.1016/j.copbio.2007.09.007
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The recent years have seen great advances in reversal of programming of differentiated somatic cells towards pluripotency by methods not involving nuclear transfer. Some of these may present a first step on the way to individual-based cell therapy without the problems connected to collection of mammalian unfertilised oocytes. Although differentiation of cells involves complex genetic and epigenetic changes, it is now possible to generate cells with many properties of pluripotent embryonic stem cells by retroviral transduction of differentiated cells with only four transcription factors: Oct3/4, Sox2, KIf4 and c-Myc. The re-programmed cells contribute to live chimeric mice and are transmitted via the germline.
引用
收藏
页码：467 / 473
页数：7
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