Cloning of cDNA for the protein disulfide isomerase from Aspergillus niger strain NNRL3 using PCR

被引：13

作者：

Malpricht, S ^{[1
]}

Thamm, A ^{[1
]}

Khanh, NQ ^{[1
]}

机构：

[1] TH DARMSTADT,INST BIOCHEM,D-64287 DARMSTADT,GERMANY

来源：

BIOTECHNOLOGY LETTERS | 1996年 / 18卷 / 04期

关键词：

D O I：

10.1007/BF00143468

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The protein disulfide isomerase from A. niger was cloned as a series of overlapping DNA-fragments generated using polymerase chain reaction technology and primers derived from conserved regions of published PDI amino acid sequences. The 5' end of the gene was amplified using inverse PCR. Comparison of amino acid sequences from rat, wheat, yeast and another fungal species shows that the thioredoxin like active sites are strongly conserved. The C-terminus of the fungal PDI contains an endoplasmatic reticulum (ER) retention signal (HDEL) that is preferred by yeast.

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页码：445 / 450

页数：6

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