Effects of bee venom on the pro-inflammatory responses in RAW264.7 macrophage cell line

被引：45

作者：

Jang, HS

Kim, SK

Han, JB

Ahn, HJ

Bae, H

Min, BI ^{[1
]}

机构：

[1] Kyung Hee Univ, Grad Sch, Dept E W Med, Seoul 130701, South Korea

[2] Kyung Hee Univ, Coll Med, Dept Microbiol & Med Sci, Seoul 130701, South Korea

[3] Kyung Hee Univ, Coll Med, Engn Res Ctr React Oxygen Species, Seoul 130701, South Korea

[4] Kyung Hee Univ, Coll Oriental Med, Dept Physiol, Seoul 130701, South Korea

[5] Kyung Hee Univ, Coll Med, Dept Physiol, Seoul 130701, South Korea

[6] Jaseng Hosp Oriental Med, Seoul, South Korea

来源：

JOURNAL OF ETHNOPHARMACOLOGY | 2005年 / 99卷 / 01期

关键词：

bee venom; nitric oxide synthase; cyclooxygenase-2; nuclear factor-kappa B; mitogen-activated protein kinase;

D O I：

10.1016/j.jep.2005.02.026

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

The purpose of this study is to elucidate the molecular mechanism of anti-inflammatory effect of bee venom (BV), which has been used for the treatment of various inflammatory diseases in oriental medicine. With this aim, we examined the effects of BV on the nitric oxide (NO) production by lipopolysaccharide (LPS) or sodium nitroprusside in RAW264.7 macrophages. We further investigated the effects of BV on the expression of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), nuclear factor-kappa B (NF-kappa B) and mitogen-activated protein kinase (MAPK) with RT-PCR in LPS-stimulated RAW264.7 cells. BV suppressed the NO production and decreased the levels of iNOS, COX-2, NF-kappa B and MAPK mRNA in a dose-dependent manner. These results suggest that BV has an anti- inflammatory effect by inhibiting iNOS and COX-2 expression, possibly through suppression of NF-kappa B and MAPK expression. (c) 2005 Elsevier Ireland Ltd. All rights reserved.

引用

页码：157 / 160

页数：4

共 21 条

[1] IκB-NF-κB structures:: At the interface of inflammation control [J].