Application of a quantum cascade laser aperture scanning near-field optical microscope to the study of a cancer cell

被引：6

作者：

Smith, Caroline I. ^{[1
]}

Siggel-King, Michele R. F. ^{[1
,2
,3
]}

Ingham, James ^{[1
]}

Harrison, Paul ^{[1
]}

Martin, David S. ^{[1
]}

Varro, Andrea ^{[4
]}

Pritchard, D. Mark ^{[4
]}

Surman, Mark ^{[2
,3
]}

Barrett, Steve ^{[1
]}

Weightman, Peter ^{[1
]}

机构：

[1] Univ Liverpool, Oliver Lodge Lab, Dept Phys, Liverpool L69 7ZE, Merseyside, England

[2] Sci Tech Daresbury, Cockcroft Inst, Warrington WA4 4AD, Cheshire, England

[3] Sci Tech Daresbury, STFC Daresbury Lab, Accelerator Sci & Technol Ctr ASTeC, Warrington WA4 4AD, Cheshire, England

[4] Univ Liverpool, Inst Translat Med, Dept Cellular & Mol Physiol, Liverpool L69 3GE, Merseyside, England

来源：

ANALYST | 2018年 / 143卷 / 24期

基金：

英国工程与自然科学研究理事会;

关键词：

BARRETTS-ESOPHAGUS; SPECTROSCOPY; RESOLUTION; ABNORMALITIES; PROBES;

D O I：

10.1039/c8an01183d

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

This work reports the first images obtained by combining an infrared aperture scanning near-field optical microscope (SNOM) with a quantum cascade laser (QCL). The future potential of this set-up is demonstrated by a preliminary study on an OE33 human oesophageal adenocarcinoma cell in which the cell is imaged at 1751 cm(-1), 1651 cm(-1), 1539 cm(-1) and 1242 cm(-1). In addition to the 1651 cm(-1) image, three other images were acquired within the Amide I band (1689 cm(-1), 1675 cm(-1) and 1626 cm(-1)) chosen to correspond to secondary structures of proteins. The four images obtained within the Amide I band show distinct differences demonstrating the potential of this approach to reveal subtle changes in the chemical composition of a cell.

引用

页码：5912 / 5917

页数：6

共 38 条

[1] New IR imaging modalities for cancer detection and for intra-cell chemical mapping with a sub-diffraction mid-IR s-SNOM
Amrania, H.
Drummond, L.
Coombes, R. C.
Shousha, S.
Woodley-Barker, L.
Weir, K.
Hart, W.
Carter, I.
Phillips, C. C.
[J]. FARADAY DISCUSSIONS, 2016, 187 : 539 - 553
[2] Asfar AS, 2013, CANCER METAST REV, V32, P623
[3] FTIR-based spectroscopic analysis in the identification of clinically aggressive prostate cancer
Baker, M. J.
Gazi, E.
Brown, M. D.
Shanks, J. H.
Gardner, P.
Clarke, N. W.
[J]. BRITISH JOURNAL OF CANCER, 2008, 99 (11) : 1859 - 1866
[4] Baker MJ, 2018, ANALYST, V143, P1735, DOI 10.1039/c7an01871a
[5] EXAMINATION OF THE SECONDARY STRUCTURE OF PROTEINS BY DECONVOLVED FTIR SPECTRA
BYLER, DM
SUSI, H
[J]. BIOPOLYMERS, 1986, 25 (03) : 469 - 487
[6] Barrett's esophagus and esophageal cancer: An overview
Conteduca, Vincenza
Sansonno, Domenico
Ingravallo, Giuseppe
Marangi, Stefania
Russi, Sabino
Lauletta, Gianfranco
Dammacco, Franco
[J]. INTERNATIONAL JOURNAL OF ONCOLOGY, 2012, 41 (02) : 414 - 424
[7] SNOM Imaging of a Crypt-Like Feature in Adenocarcinoma Associated with Barrett's Oesophagus
Craig, Timothy
Smith, Andrew D.
Holder, Gareth M.
Ingham, James
Smith, Caroline I.
Varro, Andrea
Pritchard, D. Mark
Barrett, Steve D.
Martin, David S.
Harrison, Paul
Wolski, Andrzej
Cricenti, Antonio
Luce, Marco
Surman, Mark
Chattopadhyay, Swapan
Weightman, Peter
Siggel-King, Michele R. F.
[J]. PHYSICA STATUS SOLIDI B-BASIC SOLID STATE PHYSICS, 2018, 255 (04):
[8] Very high resolution near-field chemical imaging using an infrared free electron laser
Cricenti, A
Generosi, R
Luce, M
Perfetti, P
Margaritondo, G
Talley, D
Sanghera, JS
Aggarwal, ID
Tolk, NH
[J]. PHYSICAL CHEMISTRY CHEMICAL PHYSICS, 2002, 4 (12) : 2738 - 2741
[9] Chemically resolved imaging of biological cells and thin films by infrared scanning near-field optical microscopy
Cricenti, A
Generosi, R
Luce, M
Perfetti, P
Margaritondo, G
Talley, D
Sanghera, JS
Aggarwal, ID
Tolk, NH
Congiu-Castellano, A
Rizzo, MA
Piston, DW
[J]. BIOPHYSICAL JOURNAL, 2003, 85 (04) : 2705 - 2710
[10] Local infrared microspectroscopy with subwavelength spatial resolution with an atomic force microscope tip used as a photothermal sensor
Dazzi, A
Prazeres, R
Glotin, E
Ortega, JM
[J]. OPTICS LETTERS, 2005, 30 (18) : 2388 - 2390

← 1 2 3 4 →