CryoEM reconstruction approaches to resolve asymmetric features

被引：18

作者：

Goetschius, Daniel J. ^{[1
,2
]}

Lee, Hyunwook ^{[1
,3
]}

Hafenstein, Susan ^{[1
,2
,3
]}

机构：

[1] Penn State Univ, Dept Biochem & Mol Biol, University Pk, PA 16802 USA

[2] Penn State Univ, Coll Med, Dept Med, Hershey, PA 17033 USA

[3] Penn State Univ, Huck Inst Life Sci, University Pk, PA 16802 USA

来源：

COMPLEMENTARY STRATEGIES TO UNDERSTAND VIRUS STRUCTURE AND FUNCTION | 2019年 / 105卷

基金：

美国国家卫生研究院;

关键词：

POLYMERASE COMPLEX; EM; SOFTWARE; GENOME;

D O I：

10.1016/bs.aivir.2019.07.007

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Although icosahedral viruses have highly symmetrical capsid features, asymmetric structural elements are also present since the genome and minor structural proteins are usually incorporated without adhering to icosahedral symmetry. Besides this inherent asymmetry, interactions with the host during the virus life cycle are also asymmetric. However, until recently it was impossible to resolve high resolution asymmetric features during single-particle cryoEM image processing. This review summarizes the current approaches that can be used to visualize asymmetric structural features. We have included examples of advanced structural strategies developed to reveal unique features and asymmetry in icosahedral viruses.

引用

收藏

页码：73 / 91

页数：19

相关论文

共 43 条

[1] Sampling the conformational space of the catalytic subunit of human γ-secretase [J].

Bai, Xiao-chen ;

Rajendra, Eeson ;

Yang, Guanghui ;

Shi, Yigong ;

Scheres, Sjors H. W. .

[2] PHYSICAL PRINCIPLES IN CONSTRUCTION OF REGULAR VIRUSES [J].

CASPAR, DLD ;

KLUG, A .

COLD SPRING HARBOR SYMPOSIA ON QUANTITATIVE BIOLOGY, 1962, 27 :1-&

[3] Implication for alphavirus host-cell entry and assembly indicated by a 3.5Å resolution cryo-EM structure [J].

Chen, Lihong ;

Wang, Ming ;

Zhu, Dongjie ;

Sun, Zhenzhao ;

Ma, Jun ;

Wang, Jinglin ;

Kong, Lingfei ;

Wang, Shida ;

Liu, Zaisi ;

Wei, Lili ;

He, Yuwen ;

Wang, Jingfei ;

Zhang, Xinzheng .

NATURE COMMUNICATIONS, 2018, 9

[4] Calicivirus VP2 forms a portal-like assembly following receptor engagement [J].

Conley, Michaela J. ;

McElwee, Marion ;

Azmi, Liyana ;

Gabrielsen, Mads ;

Byron, Olwyn ;

Goodfellow, Ian G. ;

Bhella, David .

NATURE, 2019, 565 (7739) :377-+

[5] Structures of Qβ virions, virus-like particles, and the Qβ-MurA complex reveal internal coat proteins and the mechanism of host lysis [J].

Cui, Zhicheng ;

Gorzelnik, Karl V. ;

Chang, Jeng-Yih ;

Langlais, Carrie ;

Jakana, Joanita ;

Young, Ry ;

Zhang, Junjie .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2017, 114 (44) :11697-11702

[6] Structure of the herpes simplex virus 1 capsid with associated tegument protein complexes [J].

Dai, Xinghong ;

Zhou, Z. Hong .

SCIENCE, 2018, 360 (6384) :47-+

[7] Assembly of complex viruses exemplified by a halophilic euryarchaeal virus [J].

De Colibus, Luigi ;

Roine, Elina ;

Walter, Thomas S. ;

Ilca, Serban L. ;

Wang, Xiangxi ;

Wang, Nan ;

Roseman, Alan M. ;

Bamford, Dennis ;

Huiskonen, Juha T. ;

Stuart, David, I .

NATURE COMMUNICATIONS, 2019, 10 (1)

[8] Scipion: A software framework toward integration, reproducibility and validation in 3D electron microscopy [J].

de la Rosa-Trevin, J. M. ;

Quintana, A. ;

del Cano, L. ;

Zaldivar, A. ;

Foche, I. ;

Gutierrez, J. ;

Gomez-Blanco, J. ;

Burguet-Castell, J. ;

Cuenca-Alba, J. ;

Abrishami, V. ;

Vargas, J. ;

Oton, J. ;

Sharov, G. ;

Vilas, J. L. ;

Navas, J. ;

Conesa, P. ;

Kazemi, M. ;

Marabini, R. ;

Sorzano, C. O. S. ;

Carazo, J. M. .

JOURNAL OF STRUCTURAL BIOLOGY, 2016, 195 (01) :93-99

[9] Correction of high-resolution data for curvature of the Ewald sphere [J].

DeRosier, DJ .

ULTRAMICROSCOPY, 2000, 81 (02) :83-98

[10]

Ewald PP, 1921, ANN PHYS-BERLIN, V64, P253

← 1 2 3 4 5 →