Antiproliferative Activity of Walnut (Juglans regia L.) Proteins and Walnut Protein Hydrolysates

被引：16
作者：
Carrillo, Wilman [1 ,2 ]
Angel Gomez-Ruiz, Jose [3 ]
Ruiz, Ana Lucia [4 ]
Carvalho, Joao Ernesto [4 ]
机构：
[1] Tech Univ Ambato, Fac Foods Sci & Engn, Lab Funct Foods, Ave Los Chasquis & Rio Payamino,Campus Huachi, Ambato 1801334, Tungurahua, Ecuador
[2] Bolivar State Univ, Fac Hlth & Human Sci, Res Dept, Guaranda, Ecuador
[3] Autonomous Univ Madrid, CSIC, Res Inst Food Sci CIAL, Cantoblanco Campus, Madrid, Spain
[4] State Univ Campinas UNICAMP, CPQBA, Paulinia, Brazil
来源：
JOURNAL OF MEDICINAL FOOD | 2017年 / 20卷 / 11期
关键词：
<bold>antiproliferative activity</bold>; <bold>cytotoxicity</bold>; <bold>hydrolysates</bold>; <bold>walnut proteins</bold>; ANTIOXIDANT PEPTIDES; ANTIMICROBIAL PEPTIDES; IDENTIFICATION; CANCER; BREAST;
D O I：
10.1089/jmf.2017.0063
中图分类号：
R914 [药物化学];
学科分类号：
100701 ;
摘要：
Proteins from Juglans regia L. were isolated. Then, proteins were hydrolyzed with different enzymes. Antiproliferative activity of proteins and of the protein hydrolysates of J. regia L. were evaluated using the sulforhodamine B method. Glutelin and prolamin proteins presented a high antiproliferative activity against cancer cells PC-3 (prostate) and K-562 (leukemia) with values of 43.9 and 84.4g/mL, respectively. The highest inhibitory effect observed was 50% at 0.25g/mL concentration in gastrointestinal digestion with pepsin and corolase pp in a dose-dependent manner against cancer cell UACC62 (melanoma). Pepsin hydrolysate showed inhibitory effects against cancer cell UACC62 (melanoma) with a concentration of 71.0g/mL. The effects were studied in a dose-dependent manner. The hydrolysate obtained with neutrase enzyme presented inhibitory effects against cancer cell UACC62 (melanoma) at a concentration of 25g/mL. Neither proteins nor protein hydrolysates presented cytotoxicity against normal cell assay VERO (epithelial).
引用
收藏
页码：1063 / 1067
页数：5
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