The effects of marine farm-scale sequentially integrated multi-trophic aquaculture systems on microbial community composition, prevalence of sulfonamide-resistant bacteria and sulfonamide resistance gene sul1

被引:31
作者
Ying, Chingwen [1 ]
Chang, Man-Jung [1 ]
Hu, Chia-Hsin [1 ]
Chang, Yi-Tang [1 ]
Chao, Wei-Liang [1 ]
Yeh, Shinn-Lih [2 ]
Chang, Su-Jung [2 ]
Hsu, Jih-Tay [3 ]
机构
[1] Soochow Univ, Dept Microbiol, Taipei 111, Taiwan
[2] Council Agr, Fisheries Res Inst, Mariculture Res Ctr, Tainan 724, Taiwan
[3] Natl Taiwan Univ, Dept Anim Sci, Taipei 111, Taiwan
关键词
Antibiotic resistance; Microbial community composition; Integrated multi-trophic aquaculture; Chanos chanos; ANTIBIOTIC-RESISTANCE; ESCHERICHIA-COLI; WATER; EFFLUX; ARGS; DISSEMINATION; ENVIRONMENTS; SEQUENCE; PONDS;
D O I
10.1016/j.scitotenv.2018.06.204
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
Aquaculture, one of the most important food production practices worldwide, faces serious challenges of mitigating the detrimental impacts of intensive farming on the environment and increased prevalence of antibiotic resistance. To develop an environment-friendly aquaculture system, a land-based and farm-scale sequentially integrated multi-trophic aquaculture (IMTA) system was established for farming Chanos chanos in southwestern Taiwan. In this system, fishes are cultured in combination with organic extractive shellfish and inorganic extractive seaweed. This study aimed to evaluate the prevalence of sulfonamide-resistant bacteria, microbial community structure, and occurrence of sulfonamide resistance genes in the IMTA and traditional aquaculture systems. Water and sediment samples were collected before raising and after harvesting C. chanos. Our results showed that the occurrence of sulfonamide-resistant phenotypes in the IMTA system was comparable with that in influent seawater, while the traditional system exhibited a high sulfonamide resistance rate. Additionally, the traditional system resulted in a deviation of the bacterial community structure from that of seawater. In the water samples from the IMTA system and influent seawater, Proteobacteria and Bacteroidetes were the two dominant phyla, representing approximately 75% and 15% of the community, respectively. In the traditional system, Actinobacteria, constituting 39% of the community, was the dominant bacterial phylum. Thirty-one sulfonamide-resistant bacterial species were isolated. In conclusion, a sequentially IMTA system showed superior ability to maintain the prevalence of antibiotic resistance and the integrity of the bacterial community structure compared to the traditional farming system, representing a potentially valuable aquaculture system for preserving the sustainability of the marine environment. (C) 2018 Elsevier B.V. All rights reserved.
引用
收藏
页码:681 / 691
页数:11
相关论文
共 51 条
  • [1] Antibiotics and antibiotic resistance in water environments
    Baquero, Fernando
    Martinez, Jose-Luis
    Canton, Rafael
    [J]. CURRENT OPINION IN BIOTECHNOLOGY, 2008, 19 (03) : 260 - 265
  • [2] Demonstrating plasmid-based horizontal gene transfer in complex environmental matrices: A practical approach for a critical review
    Bellanger, Xavier
    Guilloteau, Helene
    Bonot, Sebastien
    Merlin, Christophe
    [J]. SCIENCE OF THE TOTAL ENVIRONMENT, 2014, 493 : 872 - 882
  • [3] Environmental factors influencing the development and spread of antibiotic resistance
    Bengtsson-Palme, Johan
    Kristiansson, Erik
    Larsson, D. G. Joakim
    [J]. FEMS MICROBIOLOGY REVIEWS, 2018, 42 (01) : 68 - 80
  • [4] AMINOGLYCOSIDE ANTIBIOTIC-INACTIVATING ENZYMES IN ACTINOMYCETES SIMILAR TO THOSE PRESENT IN CLINICAL ISOLATES OF ANTIBIOTIC-RESISTANT BACTERIA
    BENVENISTE, R
    DAVIES, J
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1973, 70 (08) : 2276 - 2280
  • [5] RETRACTED: Microbial pollution in wildlife: Linking agricultural manuring and bacterial antibiotic resistance in red-billed choughs (Retracted article. See vol. 126, pg. 222, 2013)
    Blanco, Guillermo
    Lemus, Jesus A.
    Grande, Javier
    [J]. ENVIRONMENTAL RESEARCH, 2009, 109 (04) : 405 - 412
  • [6] Towards the understanding of antibiotic occurrence and transport in groundwater: Findings from the Baix Fluvia alluvial aquifer (NE Catalonia, Spain)
    Boy-Roura, M.
    Mas-Pla, J.
    Petrovic, M.
    Gros, M.
    Soler, D.
    Brusi, D.
    Mencio, A.
    [J]. SCIENCE OF THE TOTAL ENVIRONMENT, 2018, 612 : 1387 - 1406
  • [7] Ultra-high-throughput microbial community analysis on the Illumina HiSeq and MiSeq platforms
    Caporaso, J. Gregory
    Lauber, Christian L.
    Walters, William A.
    Berg-Lyons, Donna
    Huntley, James
    Fierer, Noah
    Owens, Sarah M.
    Betley, Jason
    Fraser, Louise
    Bauer, Markus
    Gormley, Niall
    Gilbert, Jack A.
    Smith, Geoff
    Knight, Rob
    [J]. ISME JOURNAL, 2012, 6 (08) : 1621 - 1624
  • [8] Prevalence of Antibiotic-Resistant Escherichia coli in Drinking Water Sources in Hangzhou City
    Chen, Zhaojun
    Yu, Daojun
    He, Songzhe
    Ye, Hui
    Zhang, Lei
    Wen, Yanping
    Zhang, Wenhui
    Shu, Liping
    Chen, Shuchang
    [J]. FRONTIERS IN MICROBIOLOGY, 2017, 8
  • [9] Chopin T., 2006, Northern Aquaculture, P4
  • [10] CLSI, 2017, PERF STAND ANT M100