Dilution and Magnification Effects on Image Analysis Applications in Activated Sludge Characterization

被引：14

作者：

Mesquita, D. P. ^{[1
]}

Dias, O. ^{[1
]}

Elias, R. A. V. ^{[2
]}

Amaral, A. L. ^{[1
,3
]}

Ferreira, E. C. ^{[1
]}

机构：

[1] Univ Minho, IBB, Ctr Biol Engn, P-4710057 Braga, Portugal

[2] Inst Politecn Braganca, Escola Super Tecnol & Gestao, P-5301857 Braganca, Portugal

[3] Inst Politecn Coimbra, Inst Super Engn Coimbra, P-3030199 Coimbra, Portugal

来源：

MICROSCOPY AND MICROANALYSIS | 2010年 / 16卷 / 05期

关键词：

activated sludge; image analysis; dilution; magnification; aggregates; filaments; ANAEROBIC GRANULAR SLUDGE; PARTIAL LEAST-SQUARES; ANALYSIS INFORMATION; FILAMENTOUS BULKING; STRUCTURAL-CHANGES; SETTLING ABILITY; DIAGNOSTIC-TOOL; PROTOZOA; PLANT; SETTLEABILITY;

D O I：

10.1017/S1431927610093785

中图分类号：

T [工业技术];

学科分类号：

08 ;

摘要：

The properties of activated sludge systems can be characterized using image analysis procedures. When these systems operate with high biomass content, accurate sludge characterization requires samples to be diluted. Selection of the best image acquisition magnification is directly related to the amount of biomass screened. The aim of the present study was to survey the effects of dilution and magnification on the assessment of aggregated and filamentous bacterial content and structure using image analysis procedures. Assessments of biomass content and structure were affected by dilutions. Therefore, the correct operating dilution requires careful consideration. Moreover, the acquisition methodology comprising a 100X magnification allowed data on aggregated and filamentous biomass to be determined and smaller aggregates to be identified and characterized, without affecting the accuracy of lower magnifications regarding biomass representativeness.

引用

页码：561 / 568

页数：8

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