Electron spin resonance analysis of superoxide anion radical scavenging activity with spin trapping agent, Diphenyl-PMPO

被引：11
|
作者：
Shimamura, Tomoko [1 ]
Fujimura, Yume [1 ]
Ukeda, Hiroyuki [1 ]
机构：
[1] Kochi Univ, Dept Bioresource Sci, Fac Agr, Nanko Ku, Kochi 7838502, Japan
来源：
ANALYTICAL SCIENCES | 2007年 / 23卷 / 10期
关键词：
D O I：
10.2116/analsci.23.1233
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
Recently, a novel electron spin resonance (ESR) spin-trapping agent, 2-(diphenylphosphinoyl)-2-methyl-3,4-dihydro-2H-pyrrole N-oxide (Diphenyl-PMPO), was synthesized. Because it had some advantages in stability and reactivity over conventional spin-trapping agents, we applied it to the ESR analyses of superoxide anion radical scavenging activity (SOSA) of superoxide dismutase (SOD) and of well-known natural antioxidants (green tea, oolong tea, and red wine). At the same time, the results with Diphenyl-PMPO were compared with 5,5-dimethyl-1-pyrroline N-oxide (DMPO). Our results revealed that Diphenyl-PMPO showed higher detectability than DMPO in the SOSA assays of SOD and natural antioxidants, so it could be available for the ESR analyses as an alternative to conventional spin-trapping agents.
引用
收藏
页码：1233 / 1235
页数：3
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