HINKEL kinesin, ANP MAPKKKs and MKK6/ANQ MAPKK, which phosphorylates and activates MPK4 MAPK, constitute a pathway that is required for cytokinesis in Arabidopsis thaliana

被引:146
作者
Takahashi, Yuji [1 ]
Soyano, Takashi [1 ]
Kosetsu, Ken [1 ]
Sasabe, Michiko [1 ]
Machida, Yasunori [1 ]
机构
[1] Nagoya Univ, Grad Sch Sci, Div Biol Sci, Chikusa Ku, Nagoya, Aichi 4648602, Japan
关键词
Arabidopsis; Cytokinesis; Kinesin-like protein; MAP kinase cascade; Phragmoplast; Tobacco; KINASE KINASE KINASE; CELL-PLATE FORMATION; PLANT CYTOKINESIS; PROTEIN; EXPANSION; ENCODES; MEKK1; GENE; NPK1; MKK2;
D O I
10.1093/pcp/pcq135
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Cytokinesis is regulated to ensure the precise partitioning of cytoplasm and duplicated chromosomes to daughter cells. The NACK-PQR pathway, which includes NACK1 kinesin-like protein (KLP) and a mitogen-activated protein kinase (MAPK) cascade, plays a key role in cytokinesis in tobacco cells. Although HINKEL/AtNACK1 (HIK) KLP, ANP MAP kinase kinase kinases (MAPKKKs) and MKK6/ANQ MAP kinase kinase (MAPKK) have been identified independently as regulators of cytokinesis in Arabidopsis thaliana, the involvement of HIK, ANPs and MKK6/ANQ in a regulatory cascade remains to be demonstrated. Here we provide details of the protein kinase pathway that controls cytokinesis in A. thaliana. Analysis of the subcellular distribution of six MAPKKs of A. thaliana that had been fused to green fluorescent protein revealed that only MKK6/ANQ protein was concentrated at the equatorial plane of the phragmoplast, at the site of localization of HIK. Expression of MKK6/ANQ in yeast cells replaced the growth-control function of the MAPKK encoded by yeast PBS2, provided that both ANP1 MAPKKK and HIK [or TETRASPORE/AtNACK2 (TES)] KLP were coexpressed, suggesting that ANP1 activates MKK6/ANQ in the presence of HIK (or TES). Coexpression of HIK and ANP3 (another member of the ANP MAPKKK family) weakly activated MKK6/ANQ but that of TES and ANP3 did not. MKK6/ANQ phosphorylated MPK4 MAPK in vitro to activate the latter kinase. Thus cytokinesis in A. thaliana is controlled by a pathway that consists of ANP MAPKKKs that can be activated by HIK and MKK6/ANQ MAPKK, with MPK4 MAPK being a probable target of MKK6/ANQ.
引用
收藏
页码:1766 / 1776
页数:11
相关论文
共 43 条
  • [1] MICROTUBULE TRANSLOCATION IN THE CYTOKINETIC APPARATUS OF CULTURED TOBACCO CELLS
    ASADA, T
    SONOBE, S
    SHIBAOKA, H
    [J]. NATURE, 1991, 350 (6315) : 238 - 241
  • [2] The cytokinesis gene KEULE encodes a Sec1 protein that binds the syntaxin KNOLLE
    Assaad, FF
    Huet, Y
    Mayer, U
    Jürgens, G
    [J]. JOURNAL OF CELL BIOLOGY, 2001, 152 (03) : 531 - 543
  • [3] NPK1, A TOBACCO GENE THAT ENCODES A PROTEIN WITH A DOMAIN HOMOLOGOUS TO YEAST BCK1, STE11, AND BYR2 PROTEIN-KINASES
    BANNO, H
    HIRANO, K
    NAKAMURA, T
    IRIE, K
    NOMOTO, S
    MATSUMOTO, K
    MACHIDA, Y
    [J]. MOLECULAR AND CELLULAR BIOLOGY, 1993, 13 (08) : 4745 - 4752
  • [4] The MAP kinase kinase MKK2 affects disease resistance in Arabidopsis
    Brader, Gunter
    Djamei, Armin
    Teige, Markus
    Palva, E. Tapio
    Hirt, Heribert
    [J]. MOLECULAR PLANT-MICROBE INTERACTIONS, 2007, 20 (05) : 589 - 596
  • [5] AN OSMOSENSING SIGNAL TRANSDUCTION PATHWAY IN YEAST
    BREWSTER, JL
    DEVALOIR, T
    DWYER, ND
    WINTER, E
    GUSTIN, MC
    [J]. SCIENCE, 1993, 259 (5102) : 1760 - 1763
  • [6] Floral dip:: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana
    Clough, SJ
    Bent, AF
    [J]. PLANT JOURNAL, 1998, 16 (06) : 735 - 743
  • [7] Involvement of MPK4 in osmotic stress response pathways in cell suspensions and plantlets of Arabidopsis thaliana:: activation by hypoosmolarity and negative role in hyperosmolarity tolerance
    Droillard, MJ
    Boudsocq, M
    Barbier-Brygoo, H
    Laurière, C
    [J]. FEBS LETTERS, 2004, 574 (1-3): : 42 - 48
  • [8] HUSH JM, 1994, J CELL SCI, V107, P775
  • [9] Ichimura K, 2002, TRENDS PLANT SCI, V7, P301, DOI 10.1016/S1360-1385(02)02302-6
  • [10] MEKK1 is required for MPK4 activation and regulates tissue-specific and temperature-dependent cell death in Arabidopsis
    Ichimura, Kazuya
    Casais, Catarina
    Peck, Scott C.
    Shinozaki, Kazuo
    Shirasu, Ken
    [J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 2006, 281 (48) : 36969 - 36976