Cell/surface interactions and adhesion on Ti-6Al-4V: Effects of surface texture

被引：54

作者：

Chen, J.

Mwenifumbo, S.

Langhammer, C.

McGovern, J.-P.

Li, M.

Beye, A.

Soboyejo, W. O. ^{[1
]}

机构：

[1] Princeton Univ, Princeton Inst Sci & Technol Mat, Princeton, NJ 08544 USA

[2] Princeton Univ, Dept Mech & Aerosp Engn, Princeton, NJ 08544 USA

[3] Spectra Phys Inc, Mountain View, CA 94043 USA

[4] Cheikh Anta Diop Univ, Dept Phys, Dakar, Senegal

来源：

JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS | 2007年 / 82B卷 / 02期

关键词：

surface texture; cell spreading and adhesion; immunofluorescence staining; enzymatic detachment;

D O I：

10.1002/jbm.b.30741

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

This paper presents the results of an experimental study of the effects of surface texture on the interactions between human osteo-sarcoma (HOS) cells and Ti-6AI-4V. These include the Ti-6AI-4V with polished (smooth); AI(2)O(3) blasted (rough); and laser micro-grooved geometries with controlled spacings and depths. Immuno-fluorescence staining of adhesion proteins (actin and vinculin) was used to study the spreading and adhesion of HOS cells in 2 day culture experiments. Quantitative measures of adhesion were also obtained using an enzymatic detachment assay. The results are discussed within the context of existing theories of cell adhesion. The implications of the results are also examined for the design of textured surfaces in biomedical systems. (C) 2007 Wiley Periodicals, Inc.

引用

页码：360 / 373

页数：14

共 78 条

[11] Substratum roughness alters the growth, area, and focal adhesions of epithelial cells, and their proximity to titanium surfaces [J].