Heterogeneity of immune cells in human atherosclerosis revealed by scRNA-Seq

被引：120

作者：

Vallejo, Jenifer ^{[1
]}

Cochain, Clement ^{[2
,3
]}

Zernecke, Alma ^{[2
]}

Ley, Klaus ^{[1
,4
]}

机构：

[1] La Jolla Inst Immunol, Div Inflammat Biol, La Jolla, CA 92037 USA

[2] Univ Hosp Wurzburg, Inst Expt Biomed, Wurzburg, Germany

[3] Univ Hosp Wurzburg, Comprehens Heart Failure Ctr, Wurzburg, Germany

[4] Univ Calif San Diego, Dept Bioengn, La Jolla, CA 92093 USA

来源：

CARDIOVASCULAR RESEARCH | 2021年 / 117卷 / 13期

关键词：

Atherosclerosis; scRNA-Seq; Transcriptomes; Antibodies; Human; T-CELLS; RECRUITMENT; MACROPHAGES; RECEPTORS; PROTEINS; SELECTIN;

D O I：

10.1093/cvr/cvab260

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Immune cells in atherosclerosis include T, B, natural killer (NK) and NKT cells, macrophages, monocytes, dendritic cells (DCs), neutrophils, and mast cells. Advances in single-cell RNA sequencing (sRNA-Seq) have refined our understanding of immune cell subsets. Four recent studies have used scRNA-Seq of immune cells in human atherosclerotic lesions and peripheral blood mononuclear cells (PBMCs), some including cell surface phenotypes revealed by oligonucleotide-tagged antibodies, which confirmed known and identified new immune cell subsets and identified genes significantly up-regulated in PBMCs from HIV+ subjects with atherosclerosis compared to PBMCs from matched HIV+ subjects without atherosclerosis. The ability of scRNA-Seq to identify cell types is greatly augmented by adding cell surface phenotype using antibody sequencing. In this review, we summarize the latest data obtained by scRNA-Seq on plaques and human PBMCs in human subjects with atherosclerosis.

引用

页码：2537 / 2543

页数：7

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