Thermophilic ethanol fermentation from lignocellulose hydrolysate by genetically engineered Moorella thermoacetica

被引：32

作者：

Rahayu, Farida ^{[1
,2
]}

Kawai, Yuto ^{[1
]}

Iwasaki, Yuki ^{[1
]}

Yoshida, Koichiro ^{[1
]}

Kita, Akihisa ^{[1
]}

Tajima, Takahisa ^{[1
]}

Kato, Junichi ^{[1
]}

Murakami, Katsuji ^{[3
]}

Hoshino, Tamotsu ^{[3
]}

Nakashimada, Yutaka ^{[1
]}

机构：

[1] Hiroshima Univ, Grad Sch Adv Sci Matter, Dept Mol Biotechnol, 1-3-1 Kagamiyama, Higashihiroshima, Hiroshima 7398530, Japan

[2] Indonesian Sweetener & Fiber Crops Res Inst, Jalan Raya Karangploso Km 9, Malang 65152, East Java, Indonesia

[3] Natl Inst Adv Ind Sci & Technol, Biomass Refinery Res Ctr, 3-11-32 Kagamiyama, Higashihiroshima, Hiroshima 7390046, Japan

来源：

BIORESOURCE TECHNOLOGY | 2017年 / 245卷

基金：

日本学术振兴会;

关键词：

Thermophilic; Acetogen; Ethanol; Moorella; Fermentation; Lignocellulosic biomass; SIMULTANEOUS SACCHARIFICATION; PRODUCE ETHANOL; HIGH-YIELD; ACETOGENS; DETOXIFICATION; TRANSFORMATION; BIOTECHNOLOGY; EXPRESSION; BACTERIUM;

D O I：

10.1016/j.biortech.2017.05.146

中图分类号：

S2 [农业工程];

学科分类号：

0828 ;

摘要：

A transformant of Moorella thermoacetica was constructed for thermophilic ethanol production from lignocellulosic biomass by deleting two phosphotransacetylase genes, pdul1 and pdul2, and introducing the native aldehyde dehydrogenase gene (aldh) controlled by the promoter from glyceraldehyde-3phosphate dehydrogenase. The transformant showed tolerance to 540 mM and fermented sugars including fructose, glucose, galactose and xylose to mainly ethanol. In a mixed-sugar medium of glucose and xylose, all of the sugars were consumed to produce ethanol at the yield of 1.9 mol/mol-sugar. The transformant successfully fermented sugars in hydrolysate prepared through the acid hydrolysis of lignocellulose to ethanol, suggesting that this transformant can be used to ferment the sugars in lignocellulosic biomass for ethanol production. (C) 2017 Elsevier Ltd. All rights reserved.

引用

页码：1393 / 1399

页数：7

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