Fluorescence-lifetime determination in tissues or other scattering media from measurement of excitation and emission kinetics

被引：42
作者：
Hutchinson, CL
Troy, TL
SevickMuraca, EM
机构：
[1] School of Chemical Engineering, Purdue University, West Lafayette, IN
来源：
APPLIED OPTICS | 1996年 / 35卷 / 13期
关键词：
D O I：
10.1364/AO.35.002325
中图分类号：
O43 [光学];
学科分类号：
070207 ; 0803 ;
摘要：
Measurements of nanosecond and subnanosecond fluorescence lifetimes are restricted to dilute, nonscattering systems since excitation and emission photon times of flight significantly affect measured fluorescent decay kinetics. We provide the theoretical rationale for frequency-domain measurements of phase-shift and amplitude demodulation made at excitation and emission wavelengths for direct determination of lifetimes in tissues and other scattering media. We confirm our analytical expressions using standard laser dyes such as 3,3'-diethylthiatricarbocyanine iodide, IR-125, and IR-140 in polystyrene suspensions with similar scattering properties as tissues. Our results have significant implication for lifetime-based spectroscopy in tissues and other scattering media. (C) 1996 Optical Society of America
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收藏
页码：2325 / 2332
页数：8
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