Pollination dynamics in a Douglas-fir seed orchard as revealed by pedigree reconstruction

被引：29

作者：

Lai, Ben Sk ^{[1
]}

Funda, Tomas ^{[1
,2
]}

Liewlaksaneeyanawin, Cherdsak ^{[1
]}

Klapste, Jaroslav ^{[1
,2
]}

Van Niejenhuis, Annette ^{[3
]}

Cook, Cathy ^{[3
]}

Stoehr, Michael U. ^{[4
]}

Woods, Jack ^{[5
]}

El-Kassaby, Yousry A. ^{[1
]}

机构：

[1] Univ British Columbia, Fac Forestry, Dept Forest Sci, Vancouver, BC V6T 1Z4, Canada

[2] Czech Univ Life Sci Prague, Fac Forestry & Wood Sci, Dept Dendrol & Forest Tree Breeding, Prague 16521 6, Czech Republic

[3] Western Forest Prod Inc, Saanich Forestry Ctr, Saanichton, BC V8M 1K1, Canada

[4] British Columbia Minist Forests & Range, Res Branch, Victoria, BC V8W 5Z2, Canada

[5] SelectSeed Co Ltd, Duncan, BC V9L 5Z2, Canada

来源：

ANNALS OF FOREST SCIENCE | 2010年 / 67卷 / 08期

基金：

加拿大自然科学与工程研究理事会;

关键词：

seed orchard; DNA fingerprinting; pedigree reconstruction; mating dynamics; Pseudotsuga menziesii; SUPPLEMENTAL MASS-POLLINATION; CROP MANAGEMENT-PRACTICES; CHLOROPLAST DNA MARKERS; MATING SYSTEM; POLLEN CONTAMINATION; REPRODUCTIVE PHENOLOGY; MICROSATELLITE MARKERS; PSEUDOTSUGA-MENZIESII; BRITISH-COLUMBIA; IMPACT;

D O I：

10.1051/forest/2010044

中图分类号：

S7 [林业];

学科分类号：

0829 ; 0907 ;

摘要：

Pollination dynamics was studied in a Douglas-fir (Pseudotsuga menziesii (Mirb.) Franco) seed orchard using 8 nuclear microsatellite markers and pedigree reconstruction. The seed orchard consisted of 49 parents (clones). Cone-crop management included bloom delay and supplemental mass pollination (SMP) using 12 internal and 4 external pollen donors. A random sample of 801 bulk seeds was genotyped for both haploid megagametophyte and corresponding diploid embryo. Using the parental population's multilocus genotypes, full pedigree reconstruction generated all the information needed to estimate the maternal, paternal, and parental reproductive success, selfing, pollen contamination, and pollination success of the 4 external pollen donors. Maternal, paternal, and parental reproductive success varied with 80% of gametes being produced by 23, 45, and 37% of the orchard's parents, respectively, resulting in a drastically reduced effective population size as compared to the census number (14 vs. 53). Selfing, pollen contamination, and aggregate SMP success (internal and external) were estimated to be 15.2, 10.4, and 15.0%, respectively. Full pedigree reconstruction was effective in unraveling the orchard's pollination dynamics and both female and male reproductive success.

引用

页码：808 / 808

页数：8

共 58 条

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