The dual role of LESION SIMULATING DISEASE 1 as a condition-dependent scaffold protein and transcription regulator

被引：37

作者：

Czarnocka, Weronika ^{[1
,2
]}

Van Der Kelen, Katrien ^{[3
,4
]}

Willems, Patrick ^{[3
,4
]}

Szechynska-Hebda, Magdalena ^{[1
,5
]}

Shahnejat-Bushehri, Sara ^{[6
,7
]}

Balazadeh, Salma ^{[6
,7
]}

Rusaczonek, Anna ^{[1
]}

Mueller-Roeber, Bernd ^{[6
,7
]}

Van Breusegem, Frank ^{[3
,4
]}

Karpinski, Stanislaw ^{[1
]}

机构：

[1] Warsaw Univ Life Sci SGGW, Fac Hort Biotechnol & Landscape Architecture, Dept Plant Genet Breeding & Biotechnol, Nowoursynowska St 159, PL-02776 Warsaw, Poland

[2] Warsaw Univ Life Sci SGGW, Fac Agr & Biol, Dept Bot, Nowoursynowska St 159, PL-02776 Warsaw, Poland

[3] Univ Ghent, Dept Plant Biotechnol & Bioinformat, Technol Pk 927, B-9052 Ghent, Belgium

[4] VIB Ctr Plant Syst Biol, Technol Pk 927, B-9052 Ghent, Belgium

[5] Polish Acad Sci, Inst Plant Physiol, Niezapominajek St 21, PL-30239 Krakow, Poland

[6] Univ Potsdam, Inst Biochem & Biol, Karl Liebknecht St 24-25, D-14476 Potsdam, Germany

[7] Max Planck Inst Mol Plant Physiol, Muhlenberg 1, D-14476 Potsdam, Germany

来源：

PLANT CELL AND ENVIRONMENT | 2017年 / 40卷 / 11期

关键词：

Arabidopsis; thaliana; dry weight; LSD1; oxidative stress; protein interaction; transcription regulation; PROGRAMMED CELL-DEATH; TANDEM AFFINITY PURIFICATION; MALE GAMETOPHYTE DEVELOPMENT; EXCESS EXCITATION-ENERGY; ARABIDOPSIS-THALIANA; GENE-EXPRESSION; SALICYLIC-ACID; LIGHT ACCLIMATION; OXIDATIVE STRESS; ADENOSINE KINASE;

D O I：

10.1111/pce.12994

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Since its discovery over two decades ago as an important cell death regulator in Arabidopsis thaliana, the role of LESION SIMULATING DISEASE 1 (LSD1) has been studied intensively within both biotic and abiotic stress responses as well as with respect to plant fitness regulation. However, its molecular mode of action remains enigmatic. Here, we demonstrate that nucleo-cytoplasmic LSD1 interacts with a broad range of other proteins that are engaged in various molecular pathways such as ubiquitination, methylation, cell cycle control, gametogenesis, embryo development and cell wall formation. The interaction of LSD1 with these partners is dependent on redox status, as oxidative stress significantly changes the quantity and types of LSD1-formed complexes. Furthermore, we show that LSD1 regulates the number and size of leaf mesophyll cells and affects plant vegetative growth. Importantly, we also reveal that in addition to its function as a scaffold protein, LSD1 acts as a transcriptional regulator. Taken together, our results demonstrate that LSD1 plays a dual role within the cell by acting as a condition-dependent scaffold protein and as a transcription regulator.

引用

页码：2644 / 2662

页数：19

共 97 条

[91] LESION SIMULATING DISEASE 1 and ENHANCED DISEASE SUSCEPTIBILITY 1 differentially regulate UV-C-induced photooxidative stress signalling and programmed cell death in Arabidopsis thaliana [J].