Exercising before protein intake allows for greater use of dietary protein-derived amino acids for de novo muscle protein synthesis in both young and elderly men

被引:231
作者
Pennings, Bart [1 ,2 ]
Koopman, Rene [1 ,2 ]
Beelen, Milou [1 ]
Senden, Joan M. G. [1 ,2 ]
Saris, Wim H. M. [3 ]
van Loon, Luc J. C. [1 ,2 ]
机构
[1] Maastricht Univ Med Ctr, Dept Human Movement Sci, NUTRIM Sch Nutr Toxicol & Metab, NL-6200 MD Maastricht, Netherlands
[2] Top Inst Food & Nutr, Wageningen, Netherlands
[3] Maastricht Univ Med Ctr, NUTRIM, Dept Human Biol, NL-6200 MD Maastricht, Netherlands
关键词
LABELED MILK PROTEIN; RESISTANCE EXERCISE; SKELETAL-MUSCLE; IN-VIVO; DOSE-RESPONSE; WHOLE-BODY; OLD MEN; LEUCINE; INGESTION; HUMANS;
D O I
10.3945/ajcn.2010.29649
中图分类号
R15 [营养卫生、食品卫生]; TS201 [基础科学];
学科分类号
100403 ;
摘要
Background: Sarcopenia seems to be attributed to a blunted muscle protein synthetic response to food intake and exercise. This blunted response could be the result of impaired protein digestion and absorption kinetics and lead to lower postprandial plasma amino acid availability. Objective: The objective was to compare in vivo dietary protein digestion and absorption kinetics and subsequent postprandial muscle protein synthesis rates at rest and after exercise between young and elderly men. Design: Young and elderly men consumed a 20-g bolus of intrinsically L-[1-C-13]phenylalanine-labeled protein at rest or after exercise. Continuous infusions with L-[ring-H-2(5)]phenylalanine were applied, and blood and muscle samples were collected to assess in vivo protein digestion and absorption kinetics and subsequent postprandial muscle protein synthesis rates. Results: Exogenous phenylalanine appearance rates expressed over time did not differ between groups. No differences were observed in plasma phenylalanine availability between the young (51 +/- 2%) and elderly (51 +/- 1%) men or between the rest (52 +/- 1%) and exercise (49 +/- 1%) conditions. Muscle protein synthesis rates calculated from the oral tracer were 0.0620 +/- 0.0065%/h and 0.0560 +/- 0.0039%/h for the rest condition and 0.0719 +/- 0.0057%/h and 0.0727 +/- 0.0040%/h for the exercise condition in young and elderly men, respectively (age effect: P = 0.62; exercise effect: P < 0.05; interaction of age and exercise: P = 0.52). Conclusions: Dietary protein digestion and absorption kinetics are not impaired after exercise or at an older age. Exercising before protein intake allows for a greater use of dietary protein-derived amino acids for de novo muscle protein synthesis in both young and elderly men. This trial was registered at clinicaltrials. gov as NCT00557388. Am J Clin Nutr 2011;93:322-31.
引用
收藏
页码:322 / 331
页数:10
相关论文
共 40 条
[1]   USE OF A HEATED SUPERFICIAL HAND VEIN AS AN ALTERNATIVE SITE FOR THE MEASUREMENT OF AMINO-ACID-CONCENTRATIONS AND FOR THE STUDY OF GLUCOSE AND ALANINE KINETICS IN MAN [J].
ABUMRAD, NN ;
RABIN, D ;
DIAMOND, MP ;
LACY, WW .
METABOLISM-CLINICAL AND EXPERIMENTAL, 1981, 30 (09) :936-940
[2]  
Alberti KGMM, 1998, DIABETIC MED, V15, P539, DOI 10.1002/(SICI)1096-9136(199807)15:7<539::AID-DIA668>3.0.CO
[3]  
2-S
[4]   Effects of aging on in vivo synthesis of skeletal muscle myosin heavy-chain and sarcoplasmic protein in humans [J].
Balagopal, P ;
Rooyackers, OE ;
Adey, DB ;
Ades, PA ;
Nair, KS .
AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM, 1997, 273 (04) :E790-E800
[5]  
BERGSTROM J, 1975, SCAND J CLIN LAB INV, V35, P606
[6]   LEUCINE AND PHENYLALANINE KINETICS DURING MIXED MEAL INGESTION - A MULTIPLE TRACER APPROACH [J].
BIOLO, G ;
TESSARI, P ;
INCHIOSTRO, S ;
BRUTTOMESSO, D ;
FONGHER, C ;
SABADIN, L ;
FRATTON, MG ;
VALERIO, A ;
TIENGO, A .
AMERICAN JOURNAL OF PHYSIOLOGY, 1992, 262 (04) :E455-E463
[7]  
BIOLO G, 1995, AM J PHYSIOL-ENDOC M, V268, pE514
[8]   An abundant supply of amino acids enhances the metabolic effect of exercise on muscle protein [J].
Biolo, G ;
Tipton, KD ;
Klein, S ;
Wolfe, RR .
AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM, 1997, 273 (01) :E122-E129
[9]  
Boirie Y, 1997, AM J CLIN NUTR, V65, P489
[10]   Acute postprandial changes in leucine metabolism as assessed with an intrinsically labeled milk protein [J].
Boirie, Y ;
Gachon, P ;
Corny, S ;
Fauquant, J ;
Maubois, JL ;
Beaufrere, B .
AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM, 1996, 271 (06) :E1083-E1091