A novel effector caspase (Caspase-3/7-1) involved in the regulation of immune homeostasis in Chinese mitten crab Eriocheir sinensis

被引：21

作者：

Qu, Chen ^{[1
,3
]}

Yang, Wen ^{[1
]}

Xu, Qingsong ^{[1
]}

Sun, Jiejie ^{[1
]}

Lu, Mengmeng ^{[1
]}

Wang, Ying ^{[1
]}

Liu, Chao ^{[1
]}

Wang, Weilin ^{[1
]}

Wang, Lingling ^{[1
,2
]}

Song, Linsheng ^{[1
,2
,3
]}

机构：

[1] Dalian Ocean Univ, Liaoning Key Lab Marine Anim Immunol, Dalian 116023, Peoples R China

[2] Qingdao Natl Lab Marine Sci & Technol, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao 266235, Peoples R China

[3] Dalian Ocean Univ, Liaoning Key Lab Marine Anim Immunol & Dis Contro, Dalian 116023, Peoples R China

来源：

FISH & SHELLFISH IMMUNOLOGY | 2018年 / 83卷

基金：

美国国家科学基金会;

关键词：

Eriocheir sinensis; Caspase; Apoptosis; LPS receptor; Immune homeostasis; OYSTER CRASSOSTREA-GIGAS; SCALLOP CHLAMYS-FARRERI; LPS BINDING-ACTIVITY; CELL-DEATH; MOLECULAR-CLONING; MEDIATED APOPTOSIS; ACTIVATION; LIPOPOLYSACCHARIDE; HEMOCYTES; RECOGNITION;

D O I：

10.1016/j.fsi.2018.09.013

中图分类号：

S9 [水产、渔业];

学科分类号：

0908 ;

摘要：

Caspases are a conserved family of cysteine proteases characterized by specificity for aspartic acid and play an essential role in cell apoptosis. In the present study, a novel effector caspase (designated as EsCaspase-3/7-1) was identified from Chinese mitten crab Eriocheir sinensis. The open reading frame of EsCaspase-3/7-1 cDNA was of 972 bp, encoding a polypeptide of 323 amino acids. EsCaspase-3/7-1 contained an N-terminal prodomain and a conservative C-terminal CASc domain, with the conserved active site "QACRG". The mRNA transcripts of EsCaspase-3/7-1 were constitutively expressed in all the examined tissues with high expression level in hemocytes, hepatopancreas and gill. The EsCaspase-3/7-1 protein was mainly distributed in the cytoplasm of hemocytes. After Aeromonas hydrophila and lipopolysaccharide (LPS) stimulations, the mRNA expression level of EsCaspase-3/7-1 in hemocytes increased significantly. The mRNA expression level of EsCaspase-3/7-1 in hemocytes was significantly up-regulated after H2O2 treatment in vitro. The recombinant EsCaspase-3/7.1 protein (rEsCaspase-3/7-1) was capable of hydrolyzing the substrate Ac-DEVD-pNA rather than Ac-YVAD-pNA and AcVEID-pNA in vitro, and exhibited binding activity to LPS. These results demonstrated that EsCaspase-3/7-1 might act as an LPS receptor, and play an important role in the regulation of immune homeostasis of E. sinensis.

引用

页码：76 / 83

页数：8

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