An Extracellular Loop of the Mannose Phosphotransferase System Component IIC Is Responsible for Specific Targeting by Class IIa Bacteriocins

被引：90

作者：

Kjos, Morten ^{[1
]}

Salehian, Zhian ^{[1
]}

Nes, Ingolf F. ^{[1
]}

Diep, Dzung B. ^{[1
]}

机构：

[1] Norwegian Univ Life Sci, Lab Microbial Gene Technol & Food Microbiol, Dept Chem Biotechnol & Food Sci, N-1432 As, Norway

来源：

JOURNAL OF BACTERIOLOGY | 2010年 / 192卷 / 22期

关键词：

SEC-DEPENDENT BACTERIOCIN; GENE-EXPRESSION SYSTEMS; LACTIS SUBSP CREMORIS; LISTERIA-MONOCYTOGENES; LACTOCOCCUS-LACTIS; HETEROLOGOUS EXPRESSION; ACID BACTERIA; PTS PERMEASE; AMINO-ACID; SAKACIN-P;

D O I：

10.1128/JB.00777-10

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Class IIa bacteriocins target a phylogenetically defined subgroup of mannose-phosphotransferase systems (man-PTS) on sensitive cells. By the use of man-PTS genes of the sensitive Listeria monocytogenes (mpt) and the nonsensitive Lactococcus lactis (ptn) species to rationally design a series of man-PTS chimeras and site-directed mutations, we identified an extracellular loop of the membrane-located protein MptC that was responsible for specific target recognition by the class IIa bacteriocins.

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收藏

页码：5906 / 5913

页数：8

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