The protein-protein interaction map of Helicobacter pylori

被引:827
作者
Rain, JC
Selig, L
De Reuse, H
Battaglia, V
Reverdy, C
Simon, S
Lenzen, G
Petel, F
Wojcik, J
Schächter, V
Chemama, Y
Labigne, A
Legrain, P
机构
[1] Hybrigen SA, F-75012 Paris, France
[2] Inst Pasteur, UnitePathogenie Bacterienne Muqueuses, F-75724 Paris 15, France
关键词
D O I
10.1038/35051615
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
With the availability of complete DNA sequences for many prokaryotic and eukaryotic genomes, and soon for the human genome itself, it is important to develop reliable proteome-wide approaches for a better understanding of protein function(1). As elementary constituents of cellular protein complexes and pathways, protein-protein interactions are key determinants of protein function. Here we have built a large-scale protein-protein interaction map of the human gastric pathogen Helicobacter pylori. We have used a high-throughput strategy of the yeast two-hybrid assay to screen 261 H. pylori proteins against a highly complex library of genome-encoded polypeptides(2). Over 1,200 interactions were identired between H. pylori proteins, connecting 46.6% of the proteome. The determination of a reliability score for every single protein-protein interaction and the identification of the actual interacting domains permitted the assignment of unannotated proteins to biological pathways.
引用
收藏
页码:211 / 215
页数:6
相关论文
共 23 条
  • [1] Genomic-sequence comparison of two unrelated isolates of the human gastric pathogen Helicobacter pylori
    Alm, RA
    Ling, LSL
    Moir, DT
    King, BL
    Brown, ED
    Doig, PC
    Smith, DR
    Noonan, B
    Guild, BC
    deJonge, BL
    Carmel, G
    Tummino, PJ
    Caruso, A
    Uria-Nickelsen, M
    Mills, DM
    Ives, C
    Gibson, R
    Merberg, D
    Mills, SD
    Jiang, Q
    Taylor, DE
    Vovis, GF
    Trost, TJ
    [J]. NATURE, 1999, 397 (6715) : 176 - 180
  • [2] The SWISS-PROT protein sequence database and its supplement TrEMBL in 2000
    Bairoch, A
    Apweiler, R
    [J]. NUCLEIC ACIDS RESEARCH, 2000, 28 (01) : 45 - 48
  • [3] A protein linkage map of Escherichia coli bacteriophage T7
    Bartel, PL
    Roecklein, JA
    SenGupta, D
    Fields, S
    [J]. NATURE GENETICS, 1996, 12 (01) : 72 - 77
  • [4] EXPRESSION OF HELICOBACTER-PYLORI UREASE GENES IN ESCHERICHIA-COLI GROWN UNDER NITROGEN-LIMITING CONDITIONS
    CUSSAC, V
    FERRERO, RL
    LABIGNE, A
    [J]. JOURNAL OF BACTERIOLOGY, 1992, 174 (08) : 2466 - 2473
  • [5] DONG ZM, 1993, J BIOL CHEM, V268, P11758
  • [6] CONSTRUCTION OF ISOGENIC UREASE-NEGATIVE MUTANTS OF HELICOBACTER-PYLORI BY ALLELIC EXCHANGE
    FERRERO, RL
    CUSSAC, V
    COURCOUX, P
    LABIGNE, A
    [J]. JOURNAL OF BACTERIOLOGY, 1992, 174 (13) : 4212 - 4217
  • [7] The future is function
    Fields, S
    [J]. NATURE GENETICS, 1997, 15 (04) : 325 - 327
  • [8] A genomic approach of the hepatitis C virus generates a protein interaction map
    Flajolet, M
    Rotondo, G
    Daviet, L
    Bergametti, F
    Inchauspé, G
    Tiollais, P
    Transy, C
    Legrain, P
    [J]. GENE, 2000, 242 (1-2) : 369 - 379
  • [9] Toward a functional analysis of the yeast genome through exhaustive two-hybrid screens
    FromontRacine, M
    Rain, JC
    Legrain, P
    [J]. NATURE GENETICS, 1997, 16 (03) : 277 - 282
  • [10] Toward a protein-protein interaction map of the budding yeast: A comprehensive system to examine two-hybrid interactions in all possible combinations between the yeast proteins
    Ito, T
    Tashiro, K
    Muta, S
    Ozawa, R
    Chiba, T
    Nishizawa, M
    Yamamoto, K
    Kuhara, S
    Sakaki, Y
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2000, 97 (03) : 1143 - 1147