Immune Responses to Pandemic H1N1 Influenza Virus Infection in Pigs Vaccinated with a Conserved Hemagglutinin HA1 Peptide Adjuvanted with CAF®01 or CDA/αGalCerMPEG

This study aimed to evaluate the immune response and protection correlates against influenza virus (IV) infection in pigs vaccinated with the novel NG34 HA1 vaccine candidate adjuvanted with either CAF (R) 01 or CDA/alpha GalCerMPEG (alpha GCM). Two groups of six pigs each were vaccinated intramuscularly twice with either NG34 + CAF (R) 01 or NG34 + CDA/alpha GCM. As controls, groups of animals (n = 6 or 4) either non-vaccinated or vaccinated with human seasonal trivalent influenza vaccine or NG34 + Freund's adjuvant were included in the study. All animal groups were challenged with the 2009 pandemic (pdm09) strain of H1N1 (total amount of 7 x 10(6) TCID50/mL) via intranasal and endotracheal routes 21 days after second vaccination. Reduced consolidated lung lesions were observed both on days three and seven post-challenge in the animals vaccinated with NG34 + CAF (R) 01, whereas higher variability with relatively more severe lesions in pigs of the NG34 + CDA/alpha GCM group on day three post-infection. Among groups, animals vaccinated with NG34 + CDA/alpha GCM showed higher viral loads in the lung at seven days post infection whereas animals from NG34 + CAF (R) 01 completely abolished virus from the lower respiratory tract. Similarly, higher IFN gamma secretion and stronger IgG responses against the NG34 peptide in sera was observed in animals from the NG34 + CAF (R) 01 group as compared to the NG34 + CDA/alpha GCM. NG34-vaccinated pigs with adjuvanted CAF (R) 01 or CDA/alpha GCM combinations resulted in different immune responses as well as outcomes in pathology and viral shedding.