MOF-Bacteriophage Biosensor for Highly Sensitive and Specific Detection of Staphylococcus aureus

被引:171
作者
Bhardwaj, Neha [1 ,2 ]
Bhardwaj, Sanjeev K. [1 ,2 ]
Mehta, Jyotsana [1 ,2 ]
Kim, Ki-Hyun [3 ]
Deep, Akash [1 ,2 ]
机构
[1] CSIR, CSIO, Chandigarh 160030, India
[2] Acad Sci & Innovat Res AcSIR CSIO, Chandigarh 160030, India
[3] Hanyang Univ, Dept Civil & Environm Engn, 222 Wangsimni Ro, Seoul 04763, South Korea
基金
新加坡国家研究基金会;
关键词
Staphylococcus aureus; bacteriophages; MOF; fluorescence; detection; METAL-ORGANIC FRAMEWORKS; SCREEN-PRINTED IMMUNOSENSOR; PATHOGEN DETECTION; CARBON NANOTUBES; DRUG-DELIVERY; MAGNETIC NANOPARTICLES; FOODBORNE PATHOGENS; RAPID DETECTION; FOOD; ENVIRONMENT;
D O I
10.1021/acsami.7b07818
中图分类号
TB3 [工程材料学];
学科分类号
0805 ; 080502 ;
摘要
To produce a sensitive and specific biosensor for Staphylococcus aureus, bacteriophages have been interfaced with a water-dispersible and environmentally stable metal-organic framework (MOF), NH2-MIL-53(Fe). The conjugation of the MOF with bacteriophages has been achieved through the use of glutaraldehyde as cross-linker. Highly sensitive detection of S. aureus in both synthetic and real samples was realized by the proposed MOF-bacteriophage' biosensor based on the photoluminescence quenching phenomena; limit of detection (31 CFU/mL) and range of detection (40 to 4 X 10(8) CFU/mL). This is the first report exploiting the use of an MOF-bacteriophage complex for the biosensing of S. aureus. The results of our study highlight that-the proposed biosensor is more sensitive than most of the previous methods while exhibiting some advanced features like specificity) regenerability, extended range of linear 'detection, and stability for long-term storage (even at room temperature).
引用
收藏
页码:33589 / 33598
页数:10
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