Structural analysis of the O-specific polysaccharide from the lipopolysaccharide of Aeromonas veronii bv. sobria strain K49

被引：5
|
作者：
Turska-Szewczuk, Anna [1 ]
Lindner, Buko [2 ]
Pekala, Agnieszka [3 ]
Palusinska-Szysz, Marta [1 ]
Choma, Adam [1 ]
Russa, Ryszard [1 ]
Holst, Otto [4 ]
机构：
[1] Marie Curie Sklodowska Univ, Dept Genet & Microbiol, PL-20033 Lublin, Poland
[2] Res Ctr Borstel, Div Immunochem, D-23845 Borstel, Germany
[3] Natl Inst Vet Res, Dept Fish Dis, PL-24100 Pulawy, Poland
[4] Res Ctr Borstel, Div Struct Biochem, D-23845 Borstel, Germany
来源：
CARBOHYDRATE RESEARCH | 2012年 / 353卷
关键词：
Aeromonas veronii; Fish pathogen; O-specific polysaccharide; Lipopolysaccharide; SOMATIC ANTIGENS; HYDROPHILA; PATHOGENICITY; PSEUDOMONADS; CHAINS; O-34;
D O I：
10.1016/j.carres.2012.03.007
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The O-specific polysaccharide obtained by mild-acid degradation of the lipopolysaccharide from Aeromonas veronii bv. sobria strain K49 was studied by sugar and methylation analyses along with H-1 and C-13 NMR spectroscopy. The sequence of the sugar residues was determined using H-1,H-1 NOESY and H-1, C-13 HMBC experiments. The O-specific polysaccharide was found to be a high molecular mass polysaccharide composed of repeating units of the structure: -> 2)-beta-D-Quip3NAc-(1 -> 3)-alpha-L-Rhap-(1 -> 3)-alpha-L-Rhap-(1 -> 2)-alpha-L-Rhap-(1 -> 3)-alpha-D-FucpNAc-(1 -> ESI MS confirmed the pentasaccharide structure of the repeating unit, as the molecular mass peaks seen in the spectrum differed by 812.34 u, a value corresponding to the calculated molecular mass of the O-unit. (C) 2012 Elsevier Ltd. All rights reserved.
引用
收藏
页码：62 / 68
页数：7
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