GENETIC DIVERSITY AND POPULATION STRUCTURE OF WILD RADISH IN EAST ASIA

被引:2
作者
Han, Q. [1 ]
Li, J. [2 ]
Shen, H. [1 ]
Sakaguchi, S. [3 ]
Isagi, Y. [4 ]
Setoguchi, H. [3 ]
机构
[1] Zaozhuang Univ, Coll Life Sci, Zaozhuang, Shandong, Peoples R China
[2] Shandong Acad Environm Sci Co Ltd, Jinan, Shandong, Peoples R China
[3] Kyoto Univ, Grad Sch Human & Environm Studies, Kyoto, Japan
[4] Kyoto Univ, Grad Sch Agr, Kyoto, Japan
来源
JOURNAL OF ANIMAL AND PLANT SCIENCES-JAPS | 2022年 / 32卷 / 04期
基金
日本科学技术振兴机构; 中国国家自然科学基金;
关键词
Wild radish; Genetic diversity; Population structure; DNA sequencing; Microsatellite loci; RAPHANUS-SATIVUS L; CULTIVATED RADISH; SSR MARKERS; POLYMORPHISM; SOFTWARE; PROGRAM; NUMBER; SITES;
D O I
10.36899/JAPS.2022.4.0515
中图分类号
S [农业科学];
学科分类号
09 ;
摘要
Wild radish (Raphanus sativus L. var. hortensis f. raphanistroides Makino) is widely distributed in East Asia; however, its genetic diversity and population structure remain poorly understood. In our study, DNA sequence and microsatellite loci were used to analyze wild radish populations across the whole East Asia. Population genetic diversity analysis showed that the East Asian wild radish populations generally had high genetic diversity (average H-O=0.448), distinct population structure, and a positive correlation between genetic distance and geographic distance. AMOVA analysis suggested the genetic diversity among individuals in populations by far exceeded the diversity among populations/groups. The outcrossing breeding system, long geographic distance between populations and gene flow from cultivated radish populations may explain the high level of genetic diversity within wild populations. Bayesian cluster analysis identified four genetic clusters in the East Asian wild radish populations, observed by DNA sequencing analysis. The possible factors would be various aspects, such as oceanic barrier, climate fluctuations, and pollinators' characteristics. This study provides a reference for the utilization of wild radish germplasm resources and the improvement of radish varieties in East Asia.
引用
收藏
页码:1110 / 1119
页数:10
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