Escherichia coli O157:H7 Curli Fimbriae Promotes Biofilm Formation, Epithelial Cell Invasion, and Persistence in Cattle

被引:17
作者
Sheng, Haiqing [1 ]
Xue, Yansong [1 ]
Zhao, Wei [1 ]
Hovde, Carolyn J. [1 ]
Minnich, Scott A. [1 ]
机构
[1] Univ Idaho, Bistate Sch Food Sci, Moscow, ID 83844 USA
基金
美国国家卫生研究院;
关键词
curli; E; coli O157; biofilm; invasion; cattle; SALMONELLA-TYPHIMURIUM; GENOME SEQUENCE; BINDING CURLI; O157-H7; STRAIN; COLONIZATION; PREVALENCE; EXPRESSION; PROTEIN; K-12;
D O I
10.3390/microorganisms8040580
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Escherichia coli O157:H7 (O157) is noninvasive and a weak biofilm producer; however, a subset of O157 are exceptions. O157 ATCC 43895 forms biofilms and invades epithelial cells. Tn5 mutagenesis identified a mutation responsible for both phenotypes. The insertion mapped within the curli csgB fimbriae locus. Screening of O157 strains for biofilm formation and cell invasion identified a bovine and a clinical isolate with those characteristics. A single base pair A to T transversion, intergenic to the curli divergent operons csgDEFG and csgBAC, was present only in biofilm-producing and invasive strains. Using site-directed mutagenesis, this single base change was introduced into two curli-negative/noninvasive O157 strains and modified strains to form biofilms, produce curli, and gain invasive capability. Transmission electron microscopy (EM) and immuno-EM confirmed curli fibers. EM of bovine epithelial cells (MAC-T) co-cultured with curli-expressing O157 showed intracellular bacteria. The role of curli in O157 persistence in cattle was examined by challenging cattle with curli-positive and -negative O157 and comparing carriage. The duration of bovine colonization with the O157 curli-negative mutant was shorter than its curli-positive isogenic parent. These findings definitively demonstrate that a single base pair stably confers biofilm formation, epithelial cell invasion, and persistence in cattle.
引用
收藏
页数:17
相关论文
共 47 条
  • [1] Curli biogenesis and function
    Barnhart, Michelle M.
    Chapman, Matthew R.
    [J]. ANNUAL REVIEW OF MICROBIOLOGY, 2006, 60 : 131 - 147
  • [2] Expression of and cytokine activation by Escherichia coli curli fibers in human sepsis
    Bian, Z
    Brauner, A
    Li, YH
    Normark, S
    [J]. JOURNAL OF INFECTIOUS DISEASES, 2000, 181 (02) : 602 - 612
  • [3] Prevalence and characteristics of Escherichia coli serotype O157:H7 and other verotoxin-producing E-coli in healthy cattle
    Blanco, M
    Blanco, JE
    Blanco, J
    Gonzalez, EA
    Mora, A
    Prado, C
    Fernandez, L
    Rio, M
    Ramos, J
    Alonso, MP
    [J]. EPIDEMIOLOGY AND INFECTION, 1996, 117 (02) : 251 - 257
  • [4] Role of Escherichia coli curli operons in directing amyloid fiber formation
    Chapman, MR
    Robinson, LS
    Pinkner, JS
    Roth, R
    Heuser, J
    Hammar, M
    Normark, S
    Hultgren, SJ
    [J]. SCIENCE, 2002, 295 (5556) : 851 - 855
  • [5] The role of type 1 and curli fimbriae of Shiga toxin-producing Escherichia coli in adherence to abiotic surfaces
    Cookson, AL
    Cooley, WA
    Woodward, MJ
    [J]. INTERNATIONAL JOURNAL OF MEDICAL MICROBIOLOGY, 2002, 292 (3-4) : 195 - 205
  • [6] Strain-Dependent Cellular Immune Responses in Cattle following Escherichia coli O157:H7 Colonization
    Corbishley, Alexander
    Ahmad, Nur Indah
    Hughes, Kirsty
    Hutchings, Michael R.
    McAteer, Sean P.
    Connelley, Timothy K.
    Brown, Helen
    Gally, David L.
    McNeilly, Tom N.
    [J]. INFECTION AND IMMUNITY, 2014, 82 (12) : 5117 - 5131
  • [7] One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products
    Datsenko, KA
    Wanner, BL
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2000, 97 (12) : 6640 - 6645
  • [8] THE EPIDEMIOLOGY OF INFECTIONS CAUSED BY ESCHERICHIA-COLI O157-H7, OTHER ENTEROHEMORRHAGIC ESCHERICHIA-COLI, AND THE ASSOCIATED HEMOLYTIC UREMIC SYNDROME
    GRIFFIN, PM
    TAUXE, RV
    [J]. EPIDEMIOLOGIC REVIEWS, 1991, 13 : 60 - 98
  • [9] Expression of two csg operons is required for production of fibronectin- and Congo red-binding curli polymers in Escherichia coli K-12
    Hammar, M
    Arnqvist, A
    Bian, Z
    Olsen, A
    Normark, S
    [J]. MOLECULAR MICROBIOLOGY, 1995, 18 (04) : 661 - 670
  • [10] THE PREVALENCE OF ESCHERICHIA-COLI O157.H7 IN DAIRY AND BEEF-CATTLE IN WASHINGTON-STATE
    HANCOCK, DD
    BESSER, TE
    KINSEL, ML
    TARR, PI
    RICE, DH
    PAROS, MG
    [J]. EPIDEMIOLOGY AND INFECTION, 1994, 113 (02) : 199 - 207