The bioorganic chemistry of transglutaminase - from mechanism to inhibition and engineering

被引：38

作者：

Keillor, Jeffrey W. ^{[1
]}

Chica, Roberto A. ^{[1
]}

Chabot, Nicolas ^{[1
]}

Vinci, Vaierio ^{[1
]}

Pardin, Christophe ^{[1
]}

Fortin, Emanuelle ^{[1
]}

Gillet, Steve M. F. G. ^{[1
]}

Nakano, Yukiko ^{[2
]}

Kaartinen, Mari T. ^{[2
]}

Pelletier, Joelle N. ^{[1
]}

Lubell, William D. ^{[1
]}

机构：

[1] Univ Montreal, Dept Chim, Montreal, PQ H3C 3J7, Canada

[2] McGill Univ, Fac Dent, Montreal, PQ H3A 2B2, Canada

来源：

CANADIAN JOURNAL OF CHEMISTRY | 2008年 / 86卷 / 04期

关键词：

transglutaminase; enzyme mechanism; enzyme inhibition; protein engineering;

D O I：

10.1139/V08-024

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Through a multidisciplinary approach comprising organic synthesis, molecular biology, and physical organic kinetic studies, we have studied the mechanism of transglutaminase-mediated transamidation. More recently, we have applied our understanding of the mechanism to the design of reversible inhibitors and affinity labels for biological application. We have also undertaken the engineering of transglutaminase for its application as a "peptide synthase". Herein, we present a brief overview of previously published work as well as recent results presented at the 2007 Merck-Frosst Centre for Therapeutic Research Award Lecture.

引用

页码：271 / 276

页数：6

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