AFLP fingerprinting:: an efficient technique for detecting genetic variation of Xanthomonas axonopodis pv. manihotis

被引:43
作者
Restrepo, S
Duque, M
Tohme, J
Verdier, V
机构
[1] Ctr Int Agr Trop, Biotechnol Unit, Cali, Colombia
[2] Inst Francais Rech Sci Dev Cooperat, ORSTOM, Genetrop, Unite Phytopathol, F-34032 Montpellier, France
来源
MICROBIOLOGY-UK | 1999年 / 145卷
关键词
AFLP; DNA fingerprinting; genetic diversity; pathogen population study;
D O I
10.1099/13500872-145-1-107
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Xanthomonas axonopodis pv. manihotis (Xam) is the causative agent of cassava bacterial blight (CBB), a worldwide disease that is particularly destructive in South America and Africa. CBB is controlled essentially through the use of resistant varieties. To develop an appropriate disease management strategy, the genetic diversity of the pathogen's populations must be assessed. Until now, the genetic diversity of Xam was characterized by RFLP analyses using ribotyping, and plasmid and genomic Xam probes. We used AFLP (amplified fragment length polymorphism), a novel PCR-based technique, to characterize the genetic diversity of Colombian Xam isolates. Six Xam strains were tested with 65 AFLP primer combinations to identify the best selective primers. Eight primer combinations were selected according to their reproducibility, number of polymorphic bands and polymorphism detected between Xam strains. Forty-seven Xam strains, originating from different Colombian ecozones, were analysed with the selected combinations. Results obtained with AFLP are consistent with those obtained with RFLP, using plasmid DNA as a probe. Some primer combinations differentiated Xam strains that were not distinguished by RFLP analyses, thus AFLP fingerprinting allowed a better definition of the genetic relationships between Xam strains.
引用
收藏
页码:107 / 114
页数:8
相关论文
共 25 条
[1]   CHARACTERIZATION OF XANTHOMONAS-CAMPESTRIS PATHOVARS BY RIBOSOMAL-RNA GENE RESTRICTION PATTERNS [J].
BERTHIER, Y ;
VERDIER, V ;
GUESDON, JL ;
CHEVRIER, D ;
DENIS, JB ;
DECOUX, G ;
LEMATTRE, M .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1993, 59 (03) :851-859
[2]  
BOUCHER CA, 1985, J GEN MICROBIOL, V131, P2449
[3]   Xanthomonas translucens from small grains: Diversity and phytopathological relevance [J].
Bragard, C ;
Singer, E ;
Alizadeh, A ;
Vauterin, L ;
Maraite, H ;
Swings, J .
PHYTOPATHOLOGY, 1997, 87 (11) :1111-1117
[4]   Comparison of outbreak and nonoutbreak Acinetobacter baumannii strains by genotypic and phenotypic methods [J].
Dijkshoorn, L ;
Aucken, H ;
GernerSmidt, P ;
Janssen, P ;
Kaufmann, ME ;
Garaizar, J ;
Ursing, J ;
Pitt, TL .
JOURNAL OF CLINICAL MICROBIOLOGY, 1996, 34 (06) :1519-1525
[5]   Gene pool similarities of potato cyst nematode populations assessed by AFLP analysis [J].
Folkertsma, RT ;
vanderVoort, JNAMR ;
deGroot, KE ;
vanZandvoort, PM ;
Schots, A ;
Gommers, FJ ;
Helder, J ;
Bakker, J .
MOLECULAR PLANT-MICROBE INTERACTIONS, 1996, 9 (01) :47-54
[6]   Genotypic and chemotaxonomic description of two subgroups in the species Aeromonas eucrenophila and their affiliation to A-encheleia and Aeromonas DNA hybridization group 11 [J].
Huys, G ;
Altwegg, M ;
Hanninen, ML ;
Vancanneyt, M ;
Vauterin, L ;
Coopman, R ;
Torck, U ;
LuthyHottenstein, J ;
Janssen, P ;
Kersters, K .
SYSTEMATIC AND APPLIED MICROBIOLOGY, 1996, 19 (04) :616-623
[7]  
Janssen P, 1996, FEMS MICROBIOL LETT, V142, P191
[8]   Evaluation of the DNA fingerprinting method AFLP as a new tool in bacterial taxonomy [J].
Janssen, P ;
Coopman, R ;
Huys, G ;
Swings, J ;
Bleeker, M ;
Vos, P ;
Zabeau, M ;
Kersters, K .
MICROBIOLOGY-UK, 1996, 142 :1881-1893
[9]   ASSESSMENT OF GENETIC DIVERSITY AND POPULATION-STRUCTURE OF XANTHOMONAS-ORYZAE PV ORYZAE WITH A REPETITIVE DNA ELEMENT [J].
LEACH, JE ;
RHOADS, ML ;
CRUZ, CMV ;
WHITE, FF ;
MEW, TW ;
LEUNG, H .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1992, 58 (07) :2188-2195
[10]   A PCR-based DNA fingerprinting technique: AFLP for molecular typing of bacteria [J].
Lin, JJ ;
Kuo, J ;
Ma, J .
NUCLEIC ACIDS RESEARCH, 1996, 24 (18) :3649-3650