One-Way Intestinal Perfusion of PVP/VA-Poloxamer 188-Curcuma longa L. Extract Solid Dispersion in Rats In Vivo and Its Effect on HSC-T6 Cell Proliferation

被引：0

作者：

Gao, Jie ^{[1
]}

Shi, Yi ^{[1
]}

Han, Yunfeng ^{[1
]}

Tang, Xi ^{[1
]}

Bi, Ruohong ^{[1
]}

Pan, Lin ^{[1
]}

Lai, Xianrong ^{[1
,2
]}

机构：

[1] Chengdu Univ Tradit Chinese Med, Sch Pharm, 1166 Liutai Ave, Chengdu 611137, Peoples R China

[2] Chengdu Univ Tradit Chinese Med, Sch Ethn Med, Chengdu 611137, Peoples R China

来源：

AAPS PHARMSCITECH | 2022年 / 23卷 / 03期

基金：

中国国家自然科学基金;

关键词：

curcuma extracts; solid dispersion; PVP; VA; poloxamer-188; in vivo intestinal absorption; HSC-T6;

D O I：

10.1208/s12249-022-02228-6

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

Turmeric was the dried rhizome of Curcuma longa L., and its extract had important pharmacological effects such as anti-tumor, cholagogic, and antioxidant. However, curcuma extract had poor water solubility and low bioavailability, which had become the main limiting factor for its clinical application. The purpose of this study was to prepare PVP/VA-Poloxamer-188-curcuma extract solid dispersion (PAP-CSD) to improve the solubility and bioavailability of the curcuma extract. The intestinal absorption mechanism of solid dispersion of this extract was studied by one-way intestinal perfusion in rats. PAP-CSD,PVP/VA-curcuma extract solid dispersion (PA-CSD) and Poloxamer-188-curcuma extract solid dispersion (P-CSD) was able to improve the intestinal absorption of the curcuma extract (P < 0.05), and PAP-CSD (combined use of two carriers) was better than that of PA-CSD and P-CSD. CCK8 method was used to investigate the effects of the curcuma extract and PAP-CSD on the proliferation of hepatic stellate cells (HSC)-T6 cells. The inhibitory effect of PAP-CSD on the proliferation of HSC-T6 cells, related to the p38 MAPK pathway, was better than that of the curcuma extract.

引用

页数：17