Sampling, metadata and DNA extraction - important steps in metagenomic studies

被引:22
作者
Felczykowska, Agnieszka [1 ]
Krajewska, Anna [1 ]
Zielinska, Sylwia [1 ]
Los, Joanna M. [1 ]
机构
[1] Univ Gdansk, Dept Mol Biol, PL-80952 Gdansk, Poland
关键词
sampling; DNA extraction; DNA purification; soil; water; wastewater; metadata; POLYMERASE-CHAIN-REACTION; WASTE-WATER TREATMENT; IN-SITU DETECTION; RAPID METHOD; MICROBIAL COMMUNITY; NUCLEIC-ACIDS; PURIFICATION PROCEDURES; REACTION AMPLIFICATION; PHYLOGENETIC ANALYSIS; BACTERIAL DIVERSITY;
D O I
10.18388/abp.2014_916
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Metagenomic studies have become increasingly popular. They allow for the estimation of biodiversity in complex populations. This diversity presents an enormous but largely unexpected genetic and biological pool and can be exploited for the recovery of novel genes, entire metabolic pathways and their products. Generally metagenomic study is a genomic analysis of organisms by direct extraction and cloning of DNA from their natural environment. The most common problems of modern metagenomics are as follows: majority of the microorganisms present in the environment cannot be cultivated by standard techniques, DNA extraction methods are not very effective, isolated DNA is contaminated with various compounds, a choice for a screening method is not obvious.
引用
收藏
页码:151 / 160
页数:10
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