Bioanalytical characterization of proteins

被引：7

作者：

Bayard, C ^{[1
]}

Lottspeich, F ^{[1
]}

机构：

[1] Max Planck Inst Biochem, D-82152 Martinsried, Germany

来源：

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2001年 / 756卷 / 1-2期

关键词：

review; food allergy; proteins;

D O I：

10.1016/S0378-4347(01)00096-2

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Allergens from the view of a protein chemist are quite normal proteins, not to distinguish from non allergenic proteins. The first task is therefore to recognize and identify the proteins responsible for the allergenic reaction. This is usually only possible if the allergenic structure is conserved during the purification procedures. For a detailed analysis of the allergenic protein modem protein chemical methods for characterization, identification, determination of posttranslational modifications and epitope characterization have to be applied. Such techniques are briefly described in this article. (C) 2001 Elsevier Science B.V. All rights reserved.

引用

收藏

页码：113 / 122

页数：10

相关论文

共 96 条

[1] IMMUNOGLOBULIN-E ANTIBODIES THAT CROSSREACT WITH VEGETABLE FOODS, POLLEN, AND HYMENOPTERA VENOM [J].

AALBERSE, RC ;

KOSHTE, V ;

CLEMENS, JGJ .

JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY, 1981, 68 (05) :356-364

[2]

Aas K, 1975, Dev Biol Stand, V29, P90

[3]

Astwood JD, 1996, J ALLERGY CLIN IMMUN, V97, P234

[4] Cross-reactivity between the major allergen from olive pollen and unrelated glycoproteins: Evidence of an epitope in the glycan moiety of the allergen [J].

Batanero, E ;

Villalba, M ;

Monsalve, RI ;

Rodriguez, R .

JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY, 1996, 97 (06) :1264-1271

[5] Characterization of Ara h 1 by two-dimensional electrophoresis immunoblot and recombinant techniques: New digestion experiments with peanuts imitating the gastrointestinal tract [J].

Becker, WM .

INTERNATIONAL ARCHIVES OF ALLERGY AND IMMUNOLOGY, 1997, 113 (1-3) :118-121

[6] Latex allergy can induce clinical reactions to specific foods [J].

Beezhold, DH ;

Sussman, GL ;

Liss, GM ;

Chang, NS .

CLINICAL AND EXPERIMENTAL ALLERGY, 1996, 26 (04) :416-422

[7]

BEIL D, 1997, LEBENSMITTELCHEMIE, V51, P90

[8] Immunological characterization of egg white allergens collected by capillary electrophoresis [J].

Besler, M ;

Steinhart, H ;

Paschke, A .

FOOD AND AGRICULTURAL IMMUNOLOGY, 1998, 10 (02) :157-160

[9] Allergenicity of hen's egg-white proteins: IgE binding of native and deglycosylated ovomucoid [J].

Besler, M ;

Steinhart, H ;

Paschke, A .

FOOD AND AGRICULTURAL IMMUNOLOGY, 1997, 9 (04) :277-288

[10] A molecular scanner to automate proteomic research and to display proteome images [J].

Binz, PA ;

Müller, M ;

Walther, D ;

Bienvenut, WV ;

Gras, R ;

Hoogland, C ;

Bouchet, G ;

Gasteiger, E ;

Fabbretti, R ;

Gay, S ;

Palagi, P ;

Wilkins, MR ;

Rouge, V ;

Tonella, L ;

Paesano, S ;

Rossellat, G ;

Karmime, A ;

Bairoch, A ;

Sanchez, JC ;

Appel, RD ;

Hochstrasser, DF .

ANALYTICAL CHEMISTRY, 1999, 71 (21) :4981-4988

← 1 2 3 4 5 6 7 8 9 10 →