Ultrastructure and immunohistochemical characterization of proteins concerned with the secretory machinery in goat ceruminous glands

被引:6
作者
Yasui, Tadashi [1 ]
Gomi, Hiroshi [1 ]
Kitahara, Taishi [1 ]
Tsukise, Azuma [1 ]
机构
[1] Nihon Univ, Coll Bioresource Sci, Dept Vet Anat, 1866 Kameino, Fujisawa, Kanagawa 2520880, Japan
来源
EUROPEAN JOURNAL OF HISTOCHEMISTRY | 2017年 / 61卷 / 03期
关键词
Ceruminous glands; goat; secretory machinery; SNARE proteins; immunohistochemistry; ER stress; ENDOPLASMIC-RETICULUM STRESS; PANCREATIC ACINAR-CELLS; APOCRINE GLANDS; ANTIMICROBIAL SUBSTANCES; HISTOCHEMICAL ANALYSIS; REGULATED EXOCYTOSIS; SNARE PROTEINS; PERIANAL SKIN; GOBLET CELLS; MEMBRANE;
D O I
10.4081/ejh.2017.2828
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
The expression of soluble N-ethylmaleimide sensitive fusion attachment protein receptor (SNARE) proteins in apocrine glands has not been fully elucidated. In addition to performing ultrastructural observation of the ceruminous glands in goats, our study focuses on the demonstration of beta-defensins, SNARE proteins and Rab3D in these glands with the use of immunohistochemical methods. The secretory cells were equipped with two types of vesicles, Golgi apparatus and abundant rough endoplasmic reticulum (ER). Additionally, in some of them, the characteristic concentric structures composed of rough ER were observed in their circum- and infranuclear parts. The expression of phosphorylated inositol requiring enzyme 1 alpha was also detected. These findings may indicate their ability to produce numerous secretory proteins and the maintenance of homeostasis in the glandular cells. Furthermore, beta-defensins were demonstrated as products of the ceruminous glands. The present investigation also revealed the presence of SNARE proteins and Rab3D. It is suggested that these proteins are concerned with the secretory machinery of this gland type.
引用
收藏
页码:222 / 230
页数:9
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