ATP-binding cassette transporters at the zebrafish blood-brain barrier and the potential utility of the zebrafish as an in vivo model

被引:11
作者
Hotz, Jordan M. [1 ]
Thomas, Joanna R. [1 ]
Katz, Emily N. [2 ]
Robey, Robert W. [1 ]
Horibata, Sachi [1 ]
Gottesman, Michael M. [1 ]
机构
[1] NCI, Lab Cell Biol, Ctr Canc Res, NIH, 37 Convent Dr,Room 2108, Bethesda, MD 20892 USA
[2] Eunice Kennedy Shriver Natl Inst Child Hlth & Hum, Zebrafish Core, Bethesda, MD 20892 USA
基金
美国国家卫生研究院;
关键词
ABC transporters; blood-brain barrier; ABCG2; P-glycoprotein; zebrafish; P-GLYCOPROTEIN; ENDOTHELIAL-CELLS; DRUG TRANSPORTERS; NATURAL-PRODUCTS; GENE FAMILY; EXPRESSION; PROTEIN; RESISTANCE; ASTROCYTES; PERICYTES;
D O I
10.20517/cdr.2021.35
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
The brain is protected from toxins by a tightly regulated network of specialized cells, including endothelial cells, pericytes, astrocyes, and neurons, known collectively as the blood-brain barrier (BBB). This selectively permeable barrier permits only the most crucial molecules essential for brain function to enter and employs a number of different mechanisms to prevent the entry of potentially harmful toxins and pathogens. In addition to a physical barrier comprised of endothelial cells that form tight junctions to restrict paracellular transport, there is an active protective mechanism made up of energy-dependent transporters that efflux compounds back into the bloodstream. Two of these ATP-binding cassette (ABC) transporters are highly expressed at the BBB: P-glycoprotein (P-gp, encoded by the ABCB1 gene) and ABCG2 (encoded by the ABCG2 gene). Although a number of in vitro and in vivo systems have been developed to examine the role that ABC transporters play in keeping compounds out of the brain, all have inherent advantages and disadvantages. Zebrafish (Danio rerio) have become a model of interest for studies of the BBB due to the similarities between the zebrafish and mammalian BBB systems. In this review, we discuss what is known about ABC transporters in zebrafish and what information is still needed before the zebrafish can be recommended as a model to elucidate the role of ABC transporters at the BBB.
引用
收藏
页码:620 / 633
页数:14
相关论文
共 84 条
  • [1] Astrocyte-endothelial interactions at the blood-brain barrier
    Abbott, NJ
    Rönnbäck, L
    Hansson, E
    [J]. NATURE REVIEWS NEUROSCIENCE, 2006, 7 (01) : 41 - 53
  • [2] Clarification of mural cell coverage of vascular endothelial cells by live imaging of zebrafish
    Ando, Koji
    Fukuhara, Shigetomo
    Izumi, Nanae
    Nakajima, Hiroyuki
    Fukui, Hajime
    Kelsh, Robert N.
    Mochizuki, Naoki
    [J]. DEVELOPMENT, 2016, 143 (08): : 1328 - 1339
  • [3] Evolution of the vertebrate ABC gene family: Analysis of gene birth and death
    Annilo, Tarmo
    Chen, Zhang-Qun
    Shulenin, Sergey
    Costantino, Julie
    Thomas, Lauren
    Lou, Hong
    Stefanov, Stefan
    Dean, Michael
    [J]. GENOMICS, 2006, 88 (01) : 1 - 11
  • [4] Pericytes regulate the blood-brain barrier
    Armulik, Annika
    Genove, Guillem
    Mae, Maarja
    Nisancioglu, Maya H.
    Wallgard, Elisabet
    Niaudet, Colin
    He, Liqun
    Norlin, Jenny
    Lindblom, Per
    Strittmatter, Karin
    Johansson, Bengt R.
    Betsholtz, Christer
    [J]. NATURE, 2010, 468 (7323) : 557 - U231
  • [5] Assessing neuroprotective P-glycoprotein activity at the blood-brain barrier in killifish (Fundulus heteroclitus) using behavioural profiles
    Bard, Shannon Mala
    Gadbois, Simon
    [J]. MARINE ENVIRONMENTAL RESEARCH, 2007, 64 (05) : 679 - 682
  • [6] Serious Ivermectin Toxicity and Human ABCB1 Nonsense Mutations
    Baudou, Eloise
    Lespine, Anne
    Durrieu, Genevieve
    Andre, Francois
    Gandia, Peggy
    Durand, Clarisse
    Cunat, Severine
    [J]. NEW ENGLAND JOURNAL OF MEDICINE, 2020, 383 (08) : 787 - 789
  • [7] Mfsd2a is critical for the formation and function of the blood-brain barrier
    Ben-Zvi, Ayal
    Lacoste, Baptiste
    Kur, Esther
    Andreone, Benjamin J.
    Mayshar, Yoav
    Yan, Han
    Gu, Chenghua
    [J]. NATURE, 2014, 509 (7501) : 507 - +
  • [8] In vitro modeling of the neurovascular unit: advances in the field
    Bhalerao, Aditya
    Sivandzade, Farzane
    Archie, Sabrina Rahman
    Chowdhury, Ekram Ahmed
    Noorani, Behnam
    Cucullo, Luca
    [J]. FLUIDS AND BARRIERS OF THE CNS, 2020, 17 (01)
  • [9] Chemical effects on dye efflux activity in live zebrafish embryos and on zebrafish Abcb4 ATPase activity
    Bieczynski, Flavia
    Burkhardt-Medicke, Kathleen
    Luquet, Carlos M.
    Scholz, Stefan
    Luckenbach, Till
    [J]. FEBS LETTERS, 2021, 595 (06) : 828 - 843
  • [10] Formation and maintenance of the BBB
    Blanchette, Marie
    Daneman, Richard
    [J]. MECHANISMS OF DEVELOPMENT, 2015, 138 : 8 - 16