Kinetic study of peroxidase-catalyzed oxidation of 1-hydroxypyrene. Development of a nanomolar hydrogen peroxide detection system

被引：6

作者：

Ivanec-Goranina, Ruta ^{[1
,2
]}

Kulys, Juozas ^{[1
,2
]}

机构：

[1] Vilnius Gediminas Tech Univ, Fac Fundamental Sci, Dept Chem & Bioengn, LT-10223 Vilnius, Lithuania

[2] Lithuania Acad Sci, Inst Biochem, LT-08662 Vilnius, Lithuania

来源：

CENTRAL EUROPEAN JOURNAL OF BIOLOGY | 2008年 / 3卷 / 03期

关键词：

1-hydroxypyrene; Amplex Red; peroxidase; oxidation;

D O I：

10.2478/s11535-008-0021-x

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Kinetics of 1-hydroxypyrene (1-HP) oxidation catalyzed with recombinant Coprinus cinereus (rCiP) and horseradish (HRP) peroxidases was investigated with a special emphasis for developing a nanomolar hydrogen peroxide (H2O2) detection system. At pH 8.0 the bimolecular constants of 1-HP oxidation with the ferryl compounds of rCiP and HRP were equal to (1.0 +/- 0.3) x 108 M-1 s(-1) and (0.6 +/- 0.2) x 108 M-1 s(-1),respectively. High bimolecular constants and fluorescence quantum yield of 1-HP (0.66) permitted detection as low as 21 nM of H2O2. To optimize the detection system 1-HP oxidation was modeled at steady-state conditions in the range pH 5.0 to pH 8.0. The 1-HP based detection system was compared with the Amplex Red system. The peroxidase-catalyzed 1-HP oxidation system was used for determination of ozone in the air.

引用

页码：224 / 232

页数：9

共 30 条

[1] pH dependence and structural interpretation of the reactions of Coprinus cinereus peroxidase with hydrogen peroxide, ferulic acid, and 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) [J].