Study of characterization and application on the binding between 5-iodouridine with HSA by spectroscopic and modeling

被引：49

作者：

Cui, Fengling ^{[1
]}

Zhang, Qiangzhai ^{[1
]}

Yan, Yinghua ^{[1
]}

Yao, Xiaojun ^{[2
]}

Qu, Guirong ^{[1
]}

Lu, Yan ^{[1
]}

机构：

[1] Henan Normal Univ, Sch Chem & Environm Sci, Key Lab Environm Pollut Control Technol Henan Pro, Xinxiang 453007, Henan, Peoples R China

[2] Lanzhou Univ, Dept Chem, Lanzhou 730000, Peoples R China

来源：

CARBOHYDRATE POLYMERS | 2008年 / 73卷 / 03期

基金：

中国国家自然科学基金;

关键词：

5-iodouridine; human serum albumin (HSA); fluorescence quenching; modeling; synchronous fluorescence; determination;

D O I：

10.1016/j.carbpol.2007.12.032

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

The binding of 5-iodouridine with human serum albumin was investigated under the simulative physiological conditions. The fluorescence spectra in combination with UV absorption and modeling method were used in the present work. A strong fluorescence quenching reaction of 5-iodouridine to HSA was observed and the quenching mechanism was suggested as static quenching procedure. The binding constants (K) at different temperatures as well as thermodynamic parameters, enthalpy change (Delta H) and entropy change (Delta S), were calculated. It showed that the hydrophobic interaction was a predominant intermolecular force in order to stabilize the complex, which was in agreement with the result of modeling study. The binding distance between 5-iodouridine and HSA was calculated on the basis of the theory of Foster energy transfer. The effects of other ions oil the binding constants were also discussed. Synchronous fluorescence spectroscopy (SFS) technique were successfully applied to determine protein in the biological samples. (C) 2008 Elsevier Ltd. All rights reserved.

引用

页码：464 / 472

页数：9

共 38 条

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