Functional amyloids in insect immune response

被引：45

作者：

Falabella, Patrizia ^{[2
]}

Riviello, Lea ^{[2
]}

Pascale, Mariarosa ^{[2
]}

Di Lelio, Ilaria ^{[1
,3
]}

Tettamanti, Gianluca ^{[4
]}

Grimaldi, Annalisa ^{[4
]}

Iannone, Carla ^{[5
]}

Monti, Maria ^{[5
]}

Pucci, Piero ^{[5
]}

Tamburro, Antonio Mario ^{[2
]}

Deeguileor, Magda ^{[4
]}

Gigliotti, Silvia ^{[1
]}

Pennacchio, Francesco ^{[3
]}

机构：

[1] CNR, Ist Genet Biofis A Buzzati Traverso, I-80131 Naples, Italy

[2] Univ Basilicata, Dipartimento Biol Difesa & Biotecnol Agroforestal, I-85100 Potenza, Italy

[3] Univ Naples Federico II, Dipartimento Entomol & Zool Agr F Silvestri, I-80055 Naples, Italy

[4] Univ Insubria, Dipartimento Biotecnol & Sci Vita, I-21100 Varese, Italy

[5] Univ Naples Federico II, CEINGE Biotecnol Avanzate, I-80145 Naples, Italy

来源：

INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY | 2012年 / 42卷 / 03期

关键词：

Amyloids; Melanin synthesis; Cellular immunity; Humoural immunity; Haemocytes; DROSOPHILA; HEMOCYTES; MELANIZATION; PROTEINS;

D O I：

10.1016/j.ibmb.2011.11.011

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The innate immune system of insects consists of humoural and cellular responses that provide protection against invading pathogens and parasites. Defence reactions against these latter include encapsulation by immune cells and targeted melanin deposition, which is usually restricted to the surface of the foreign invader, to prevent systemic damage. Here we show that a protein produced by haemocytes of Heliothis virescens (Lepidoptera, Noctuidae) larvae, belonging to XendoU family, generates amyloid fibrils, which accumulate in large cisternae of the rough endoplasmic reticulum and are released upon immune challenge, to form a layer coating non-self objects entering the haemocoel. This amyloid layer acts as a molecular scaffold that promotes localised melanin synthesis and the adhesion of immune cells around the non-self intruder during encapsulation response. Our results demonstrate a new functional role for these protein aggregates that are commonly associated with severe human diseases. We predict that insects will offer new powerful experimental systems for studying inducible amyloidogenesis, which will likely provide fresh perspectives for its prevention. (C) 2011 Elsevier Ltd. All rights reserved.

引用

页码：203 / 211

页数：9

共 40 条

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