Ammonium transformed into nitrous oxide via nitric oxide by Pseudomonas putida Y-9 under aerobic conditions without hydroxylamine as intermediate

被引：55

作者：

Huang, Xuejiao ^{[1
]}

Xu, Yi ^{[1
]}

He, Tengxia ^{[1
]}

Jia, Hongjie ^{[1
]}

Feng, Mi ^{[1
]}

Xiang, Shudi ^{[1
]}

Wang, Shutong ^{[1
]}

Ni, Jiupai ^{[1
]}

Xie, Deti ^{[1
]}

Li, Zhenlun ^{[1
]}

机构：

[1] Southwest Univ, Chongqing Key Lab Soil Multiscale Interfacial Pro, Chongqing 400716, Peoples R China

来源：

BIORESOURCE TECHNOLOGY | 2019年 / 277卷

关键词：

Pseudomonas putida; Aerobic; Hydroxylamine transform pathway; Ammonium oxidation pathway; HETEROTROPHIC NITRIFICATION; DENITRIFICATION; MARINE; REMOVAL; AMOA; GENES; PHYLOGENY; DIVERSITY; N2O; N-2;

D O I：

10.1016/j.biortech.2019.01.040

中图分类号：

S2 [农业工程];

学科分类号：

0828 ;

摘要：

Previous studies have reported that hydroxylamine (NH2OH) is an inevitable intermediate of the ammonium (NH4+) oxidation pathway under aerobic conditions. In this study, Pseudomonas putida Y-9 was found to oxidize ammonium into N2O via NO without the accumulation of NH2OH and NO2- under aerobic conditions. NH2OH was nearly completely transformed into NO2- whether NH4+ was present in the medium, and NH4+ could accelerate the transformation of NH2OH to NO2- by promoting Y-9 growth. NH4+ was oxidized rapidly by Y-9 with or without the presence of NH2OH in the medium, and the decrease of total nitrogen reached 30.65 mg/L and 39.38 mg/L, respectively, which indicates that NH2OH inhibits the transformation efficiency of NH4+ to N2O. Gene amplification and enzyme assays demonstrated that ammonia monooxygenase doesn't exist in Y-9. All results show that NH4+ can be transformed into N2O via NO by Y-9 under aerobic conditions without NH2OH as intermediate.

引用

页码：87 / 93

页数：7

共 36 条

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