Primary capture of cyclodextrin glycosyltransferase derived from Bacillus cereus by aqueous two phase system

被引：38

作者：

Ng, Hui Suan ^{[2
]}

Tan, Chin Ping ^{[3
]}

Chen, Soo Kien ^{[4
]}

Mokhtar, Mohd Noriznan ^{[2
]}

Ariff, Arbakariya ^{[5
]}

Ling, Tau Chuan ^{[1
]}

机构：

[1] Univ Malaya, Fac Sci, Inst Biol Sci, Kuala Lumpur 50603, Malaysia

[2] Univ Putra Malaysia, Fac Engn, Dept Proc & Food Engn, Upm Serdang 43400, Selangor, Malaysia

[3] Univ Putra Malaysia, Fac Food Sci & Technol, Dept Food Technol, Upm Serdang 43400, Selangor, Malaysia

[4] Univ Putra Malaysia, Fac Sci, Dept Phys, Upm Serdang 43400, Selangor, Malaysia

[5] Univ Putra Malaysia, Fac Biotechnol & Biomol Sci, Dept Bioproc Technol, Upm Serdang 43400, Selangor, Malaysia

来源：

SEPARATION AND PURIFICATION TECHNOLOGY | 2011年 / 81卷 / 03期

关键词：

Aqueous two phase system; Cyclodextrin glycosyltransferase; Cyclodextrin; Enzyme; Purification; PENICILLIN ACYLASE; PURIFICATION;

D O I：

10.1016/j.seppur.2011.07.039

中图分类号：

TQ [化学工业];

学科分类号：

0817 ;

摘要：

In this works, the polymer-salt aqueous two phase system (ATPS) which is polyethylene-glycol (PEG) with sodium citrate was constructed to purify the enzyme CGTase from fermentation broth. Impacts of parameters such as phase composition, tie-line length (TLL), volume ratio (V-R), crude sample loading, pH and the addition of sodium chloride (NaCl) on the partition behavior of cyclodextrin glycosyltransferase (CGTase) were investigated. The study exhibited that the optimum system for the purification of the enzyme CGTase was achieved on the 19.0% PEG and 11.5% citrate system with ILL of 38.89% (w/w), V-R of 2.0, with additional 4% (w/w) NaCl and 20% crude load at pH 7.0. CGTase from Bacillus sp. was partially purified by the ATPS up to 16.3-fold with a yield of 70%. (C) 2011 Elsevier B.V. All rights reserved.

引用

页码：318 / 324

页数：7

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