LC-MS/MS assay of ropinirole in rat biological matrices: elimination of lysoglycerophosphocholines-based matrix effect

被引：5

作者：

Yan, Xingjin ^{[1
,2
]}

Zhao, Shouwei ^{[1
]}

Zhu, Wenwen ^{[1
]}

Rao, Jing ^{[1
]}

Kang, Li ^{[1
]}

Xu, Jiaqiang ^{[1
]}

Li, Dan ^{[1
]}

Qin, Lixia ^{[1
]}

Qiu, Lihui ^{[1
]}

Li, Gao ^{[1
,3
]}

Huang, Jiangeng ^{[1
,3
]}

Si, Luqin ^{[1
,3
]}

机构：

[1] Huazhong Univ Sci & Technol, Tongji Med Coll, Sch Pharm, Dept Pharmaceut, Wuhan 430030, Hubei, Peoples R China

[2] Chongqing Gen Hosp, Dept Pharm, Chongqing 400000, Peoples R China

[3] Huazhong Univ Sci & Technol, Hubei Engn Res Ctr Novel Drug Delivery Syst, Wuhan 430030, Hubei, Peoples R China

来源：

BIOANALYSIS | 2016年 / 8卷 / 17期

关键词：

intranasal administration; LC-MS/MS; matrix effect; microdialysis; ropinirole; IN-VIVO MICRODIALYSIS; PARKINSONS-DISEASE; HUMAN PLASMA; DOUBLE-BLIND; L-DOPA; BRAIN; PHARMACOKINETICS; DELIVERY; BLOOD; HYDROCHLORIDE;

D O I：

10.4155/bio-2016-0087

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Aim: To adequately support PK evaluation of ropinirole in rats following intranasal administration, it was desirable to determine ropinirole concentrations in rat plasma, brain homogenate and microdialysate. Results & methodology: A robust LC-MS/MS method has been developed for the determination of ropinirole in biological samples. Liquid-liquid extraction using ethyl acetate eliminated matrix effect due to significantly reduced levels of lysoglycerophosphocholines. The assay was fully validated with dynamic ranges of 0.01-20 ng/ml for plasma and brain homogenate samples and 0.1-200 ng/ml for microdialysate samples. Conclusion: The proposed method was accurate and precise for the quantification of ropinirole in biological samples and was successfully applied to a microdialysis study of ropinirole in rats.

引用

页码：1823 / 1835

页数：13

共 37 条

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